FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238456
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11240.471365786560204No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG11160.4680108699298823No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10460.438655349414567No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9990.41894521421142683No Hit
CCTACCGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA9680.4059449122689301No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9430.3954607977991747No Hit
ATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTCTCCATGC8760.36736337102023015No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA8280.34723387123829974No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG8130.3409434025564465No Hit
TAGACAAAGGCATCAAACCTTATTATCCGGAACATGCAGTTAATCATTAC7600.31871707988056497No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAACAGACCGCCGTG6500.2725869762136411No Hit
GTACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6290.26378032005904656No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6270.26294159090146607No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6270.26294159090146607No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6040.2532962055892911No Hit
AGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTT6000.25161874727413025No Hit
CTCCTACCGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCG5570.2335860703861509No Hit
GCTGGTATTCTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGG5540.23232797664978025No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTGTTGATGATATCCTCCTGC5480.22981178917703896No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5360.22477941423155634No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5210.21848894554970308No Hit
GTATACATGCATACAAAGGCATTAATGCAGGATAGCCACATTGTGTAAAA5160.21639212265575203No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5070.21261784144664006No Hit
ACTGACAACAGTGCCAGCAGCACCTCCTCCTGCCACCACCAATCGGCAGT4970.20842419565873788No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4940.20716610192236723No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA4650.19500452913745095No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4580.1920689770859194No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4550.19081088334954877No Hit
GATTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4510.1891334250343879No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4380.18368168551011507No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4360.18284295635253464No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4300.18032676887979332No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4300.18032676887979332No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA4160.1744556647767303No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4040.1694232898312477No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG3900.16355218572818464No Hit
ATCCTAATCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAACCTTAT3830.16061663367665313No Hit
CTTTATCTAAGGGCAAATATTTGGTAAGATTAGGATAGAATCTAGCAGGC3790.15893917536149227No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3770.15810044620391184No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3710.15558425873117054No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATAT3700.15516489415238033No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3660.15348743583721944No Hit
CTCAAGGACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3650.15306807125842922No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3650.15306807125842922No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3650.15306807125842922No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3600.15097124836447814No Hit
CTTTTACACAATGTGGCTATCCTGCATTAATGCCTTTGTATGCATGTATA3600.15097124836447814No Hit
GTCCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC3570.1497131546281075No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA3560.14929379004931728No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC3560.14929379004931728No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3550.14887442547052704No Hit
CCAAAAGACACCAAATACTCAAGGACAGTATCTCTTCCAAAAGTAAGACA3540.14845506089173685No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG3520.1476163317341564No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCAGCAAATGCC3480.14593887341899553No Hit
GGTCTTGCATGGTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3470.14551950884020534No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3350.1404871338947227No Hit
GCCTGAGGATGACTGTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3330.1396484047371423No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTATCTCTTCCAAAAGTAAGA3280.1375515818431912No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAACTGAAAGCCAAA3240.13587412352803033No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTA3220.1350353943704499No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3210.13461602979165965No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGTGGCAGGAGGAGGTGC3210.13461602979165965No Hit
GAGGATGACTGTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCT3180.133357936055289No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3160.1325192068977086No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3150.13209984231891836No Hit
CCCCAACAGACCGCCGTGTGGTGGGGTGAACCCTGGGCCGAATGCTCCCA3140.13168047774012814No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3130.13126111316133796No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA3130.13126111316133796No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3110.1304223840037575No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3060.12832556110980642No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATACAGGCCCCT3050.1279061965310162No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3030.12706746737343577No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3010.12622873821585534No Hit
GGCCTACATATTGTTGACATCTGTTGATGATATCCTCCTGCAAATGAATG3010.12622873821585534No Hit
GAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGC3010.12622873821585534No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC2990.1253900090582749No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2990.1253900090582749No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT2980.1249706444794847No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2970.12455127990069446No Hit
GGTGAACCCTGGGCCGAATGCTCCCACTCCTACCTGATTTGCCTCTGGCC2960.12413191532190425No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT2960.12413191532190425No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG2910.12203509242795316No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACC2900.12161572784916296No Hit
CCTCTAAGGGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT2890.12119636327037273No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2880.12077699869158251No Hit
GTGCACACGGTCCGGCGGATGAGAAGGCACAGACGGGGAGACCGCGTAAA2870.12035763411279231No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCGGATGAGAAGGCACAG2830.11868017579763142No Hit
GTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACACGCAGCGCCTCAT2800.11742208206126077No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT2800.11742208206126077No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG2790.11700271748247057No Hit
AGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA2780.11658335290368035No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAA2770.11616398832489012No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2730.11448653000972926No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT2730.11448653000972926No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC2730.11448653000972926No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG2690.11280907169456839No Hit
CTCCCACTCCTACCTGATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTG2690.11280907169456839No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG2690.11280907169456839No Hit
GTCCAAGAGTCCGCTTATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTC2680.11238970711577817No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC2680.11238970711577817No Hit
CTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACACGCAGCGCC2660.11155097795819773No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG2660.11155097795819773No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG2650.11113161337940752No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2640.11071224880061731No Hit
TACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACACGC2640.11071224880061731No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT2640.11071224880061731No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2630.11029288422182708No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG2620.10987351964303688No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2580.10819606132787601No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT2560.10735733217029557No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2550.10693796759150537No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2530.10609923843392492No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2530.10609923843392492No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC2520.1056798738551347No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC2490.10442178011876403No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2470.10358305096118361No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG2450.10274432180360318No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACATCCTAGCAGCC2450.10274432180360318No Hit
ATGTGGCTATCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC2440.10232495722481297No Hit
ACCCTGGGCCGAATGCTCCCACTCCTACCTGATTTGCCTCTGGCCATTGA2440.10232495722481297No Hit
GGAAAATACAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT2430.10190559264602274No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2410.10106686348844232No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC2390.10022813433086188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)