FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238456
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10750.4508169221994833No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9620.4034287247961888No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9540.4000738081658671No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9140.3832992250142584No Hit
CCTACCGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA8640.36233099607474756No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8360.35058878786862147No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG7870.33003992350790085No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA7660.32123326735330626No Hit
ATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTCTCCATGC7130.2990069446774247No Hit
TAGACAAAGGCATCAAACCTTATTATCCGGAACATGCAGTTAATCATTAC6060.25413493474687154No Hit
CTCCTACCGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCG5970.2503606535377596No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5840.24490891401348674No Hit
GTACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5530.23190861207099006No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTGTTGATGATATCCTCCTGC5450.22855369544066828No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5440.22813433086187807No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5420.22729560170429763No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5370.22519877881034658No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5180.21723085181333243No Hit
GTATACATGCATACAAAGGCATTAATGCAGGATAGCCACATTGTGTAAAA5150.21597275807696178No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAACAGACCGCCGTG5140.21555339349817157No Hit
GCTGGTATTCTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGG5060.21219847686784984No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4770.20003690408293354No Hit
GATTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4700.19710135203140203No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA4690.1966819874526118No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4550.19081088334954877No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4470.187455966719227No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA4360.18284295635253464No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4320.18116549803737378No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG4190.17571375851310095No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4110.17235884188277922No Hit
ACTGACAACAGTGCCAGCAGCACCTCCTCCTGCCACCACCAATCGGCAGT4100.17193947730398898No Hit
AGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTT4020.16858456067366726No Hit
ATCCTAATCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAACCTTAT3930.16481027946455531No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3850.16145536283423356No Hit
CTTTTACACAATGTGGCTATCCTGCATTAATGCCTTTGTATGCATGTATA3840.16103599825544335No Hit
GTCCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC3830.16061663367665313No Hit
CTTTATCTAAGGGCAAATATTTGGTAAGATTAGGATAGAATCTAGCAGGC3800.15935853994028248No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3710.15558425873117054No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3670.15390680041600965No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTATCTCTTCCAAAAGTAAGA3640.152648706679639No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3560.14929379004931728No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC3530.14803569631294664No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3510.1471969671553662No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCAGCAAATGCC3490.14635823799778575No Hit
CTCAAGGACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3450.14468077968262488No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG3350.1404871338947227No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3330.1396484047371423No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3320.13922904015835205No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA3300.13839031100077165No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3280.1375515818431912No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA3190.13377730063407925No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACC3180.133357936055289No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3120.1308417485825477No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3100.1300030194249673No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT3090.12958365484617707No Hit
GAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGC3090.12958365484617707No Hit
GCCTGAGGATGACTGTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3070.12874492568859663No Hit
CCAAAAGACACCAAATACTCAAGGACAGTATCTCTTCCAAAAGTAAGACA3020.12664810279464556No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3010.12622873821585534No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2970.12455127990069446No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC2970.12455127990069446No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2950.12371255074311402No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2950.12371255074311402No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2930.1228738215855336No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2910.12203509242795316No Hit
GGTGAACCCTGGGCCGAATGCTCCCACTCCTACCTGATTTGCCTCTGGCC2890.12119636327037273No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2850.11951890495521186No Hit
GGCCTACATATTGTTGACATCTGTTGATGATATCCTCCTGCAAATGAATG2850.11951890495521186No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA2830.11868017579763142No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT2820.11826081121884122No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTA2800.11742208206126077No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAACTGAAAGCCAAA2790.11700271748247057No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG2780.11658335290368035No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG2780.11658335290368035No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTTCCTGTAA2770.11616398832489012No Hit
CCTCTAAGGGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT2740.11490589458851948No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC2730.11448653000972926No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2710.11364780085214882No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATAT2690.11280907169456839No Hit
CTCCCACTCCTACCTGATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTG2680.11238970711577817No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC2670.11197034253698795No Hit
CCCCAACAGACCGCCGTGTGGTGGGGTGAACCCTGGGCCGAATGCTCCCA2660.11155097795819773No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2650.11113161337940752No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG2600.10903479048545643No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA2590.10861542590666622No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG2530.10609923843392492No Hit
CTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACACGCAGCGCC2520.1056798738551347No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC2510.10526050927634448No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG2510.10526050927634448No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA2500.10484114469755428No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2500.10484114469755428No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG2470.10358305096118361No Hit
GTGCACACGGTCCGGCGGATGAGAAGGCACAGACGGGGAGACCGCGTAAA2450.10274432180360318No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATACAGGCCCCT2440.10232495722481297No Hit
GAGGATGACTGTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCT2420.10148622806723252No Hit
GCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACAACGACG2400.10064749890965209No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT2400.10064749890965209No Hit
GCCTACTCCCATCTCTCCACCTCTAAGGGACAGTCATCCTCAGGCCATGC2390.10022813433086188No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2390.10022813433086188No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC2390.10022813433086188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)