FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002490108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89654
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8330.929127534744685No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7460.8320878042251323No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACCCGTAGCGCC7300.8142414170031455No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5100.5688535927008276No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5040.5621611974925826No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC3960.441698083744172No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2890.32235036919713567No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2820.31454257478751646No Hit
GGATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC2650.2955807883641555No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG2590.28888839315591053No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2480.27661900194079464No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC2320.25877261471880786No Hit
GGCAACATACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG2320.25877261471880786No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG2310.2576572155174337No Hit
GATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC2300.2565418163160595No Hit
TCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG2130.2375800298926986No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2120.23646463069132442No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2040.22754143708033106No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAAT2010.2241952394762085No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1940.2163874450665893No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC1920.214156646663841No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC1870.20857965065697012No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1810.2018872554487251No Hit
GTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1750.19519486024048008No Hit
GTATATTGCCCGTCTGTCCTCTCATTCCAGGATCTTCAACCACCAGCACG1680.18738706583086087No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC1650.18404086822673835No Hit
GGCCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAG1620.18069467062261582No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC1620.18069467062261582No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA1610.17957927142124167No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT1600.17846387221986748No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1570.17511767461574498No Hit
CTACTAGGTCCCTCGATGCTTGATCTTCCAAATTACCACCCACCCAAGTA1530.17065607781024827No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGT1530.17065607781024827No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATGAGAGGACAGACGGGCAAC1530.17065607781024827No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC1520.16954067860887412No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1510.16842527940749993No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGTCTCTA1510.16842527940749993No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT1500.16730988020612578No Hit
GCCTTAGAGTCTTCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA1500.16730988020612578No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC1490.16619448100475162No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1450.1617328841992549No Hit
ACCTAGTAGTGAGTTATGTCAACACTAATATGGGCCTAAAGTTTAGGCAA1450.1617328841992549No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT1440.16061748499788073No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA1430.15950208579650657No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA1420.15838668659513241No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT1410.15727128739375823No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT1410.15727128739375823No Hit
GGATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC1400.15615588819238407No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG1370.15280969058826152No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC1370.15280969058826152No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG1350.1505788921855132No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC1330.14834809378276487No Hit
TTATATAAGAGAGAAACAACCCGTAGCGCCTCATTTTGTGGGTCACCATA1310.1461172953800165No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1300.14500189617864231No Hit
CTACGCCTCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC1270.14165569857451982No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG1260.14054029937314566No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC1260.14054029937314566No Hit
TCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA1250.13942490017177148No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG1240.13830950097039732No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATCTTGCAGAGT1240.13830950097039732No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG1240.13830950097039732No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT1210.1349633033662748No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT1180.13161710576215227No Hit
GTTCATATATTGTGTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA1180.13161710576215227No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG1170.1305017065607781No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT1170.1305017065607781No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG1170.1305017065607781No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA1150.12827090815802974No Hit
GGGATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTC1150.12827090815802974No Hit
ATATAAGAGAGAAACAACCCGTAGCGCCTCATTTTGTGGGTCACCATATT1110.12380931135253306No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCACCTTGAGTCCCTTTTTACC1110.12380931135253306No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG1110.12380931135253306No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATCGA1110.12380931135253306No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1090.12157851294978474No Hit
GTGTAAATGTATATTAGGAAATGAGGGTGTTTTCCAATGAGGGTTAAAGA1090.12157851294978474No Hit
GGTATAGCCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTCAG1080.12046311374841055No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC1070.1193477145470364No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC1070.1193477145470364No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG1070.1193477145470364No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCCTGTATT1070.1193477145470364No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1040.11600151694291386No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACGAGATCTTCTAGATACC1020.11377071854016553No Hit
GGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG970.10819372253329465No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC960.1070783233319205No Hit
GAGTAACCCCATCTTTTAGTTTTGTCAGGGTTTAAATGTATACCCAAAGA950.10596292413054634No Hit
GTGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTAT950.10596292413054634No Hit
GGGTAAAGGTTCAGATATTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGA950.10596292413054634No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT940.10484752492917215No Hit
TCAACCACCAGCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGG940.10484752492917215No Hit
GGCTATACCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA940.10484752492917215No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT920.1026167265264238No Hit
ATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGCCTGTGCCAAG920.1026167265264238No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA910.10150132732504964No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG900.10038592812367546No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG900.10038592812367546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)