FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002490109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89654
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7300.8142414170031455No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6920.771856247350927No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACCCGTAGCGCC6420.7160862872822183No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5810.6480469359983938No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4780.5331608182568541No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC3630.40488991009882436No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3140.35023534923148997No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3060.34131215562049655No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC2440.2721574051352979No Hit
GATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC2280.2543110179133112No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2190.24427242510094363No Hit
GGATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC2190.24427242510094363No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG2120.23646463069132442No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2090.2331184330872019No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC2040.22754143708033106No Hit
GGCAACATACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG2030.22642603787895688No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC1920.214156646663841No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1920.214156646663841No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAAT1910.2130412474624668No Hit
TCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG1800.20077185624735092No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA1760.19631025944185426No Hit
GTATATTGCCCGTCTGTCCTCTCATTCCAGGATCTTCAACCACCAGCACG1730.19296406183773174No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG1730.19296406183773174No Hit
GTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1710.19073326343498337No Hit
CTACTAGGTCCCTCGATGCTTGATCTTCCAAATTACCACCCACCCAAGTA1670.1862716666294867No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG1620.18069467062261582No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC1610.17957927142124167No Hit
GCCTTAGAGTCTTCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA1600.17846387221986748No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT1580.17623307381711914No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT1570.17511767461574498No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG1540.17177147701162246No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1460.16284828340062907No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG1450.1617328841992549No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT1430.15950208579650657No Hit
ACCTAGTAGTGAGTTATGTCAACACTAATATGGGCCTAAAGTTTAGGCAA1390.15504048899100986No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1370.15280969058826152No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1370.15280969058826152No Hit
ATATAAGAGAGAAACAACCCGTAGCGCCTCATTTTGTGGGTCACCATATT1360.15169429138688736No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG1340.14946349298413902No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT1330.14834809378276487No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC1320.14723269458139068No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG1320.14723269458139068No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT1320.14723269458139068No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT1310.1461172953800165No Hit
GTGTAAATGTATATTAGGAAATGAGGGTGTTTTCCAATGAGGGTTAAAGA1310.1461172953800165No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC1300.14500189617864231No Hit
GGCCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAG1290.14388649697726816No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATGAGAGGACAGACGGGCAAC1270.14165569857451982No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG1250.13942490017177148No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCCTGTATT1250.13942490017177148No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG1220.13607870256764895No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1220.13607870256764895No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC1220.13607870256764895No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCACCTTGAGTCCCTTTTTACC1210.1349633033662748No Hit
GGATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC1190.13273250496352645No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATCTTGCAGAGT1190.13273250496352645No Hit
CTACGCCTCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC1180.13161710576215227No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC1170.1305017065607781No Hit
GGGTAAAGGTTCAGATATTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGA1170.1305017065607781No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC1140.1271555089566556No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGTCTCTA1140.1271555089566556No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA1140.1271555089566556No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATCGA1120.12492471055390725No Hit
GTTCATATATTGTGTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA1090.12157851294978474No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGT1090.12157851294978474No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA1080.12046311374841055No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT1070.1193477145470364No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1060.11823231534566221No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC1060.11823231534566221No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG1030.1148861177415397No Hit
GGTATAGCCCATAAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTCAG1010.11265531933879135No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1010.11265531933879135No Hit
TCAACCACCAGCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGG1000.11153992013741719No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC990.110424520936043No Hit
CTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACCCGTAGCGCCTC980.10930912173466883No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG980.10930912173466883No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG980.10930912173466883No Hit
TTATATAAGAGAGAAACAACCCGTAGCGCCTCATTTTGTGGGTCACCATA970.10819372253329465No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT970.10819372253329465No Hit
GCTTTAATGCCCTTATATGCCTGTATTCAATCTCGTCAGGCTTTCACTTT960.1070783233319205No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTATATGCCTGTATTCAATCTCGTC960.1070783233319205No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA950.10596292413054634No Hit
ATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGCCTGTGCCAAG950.10596292413054634No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT940.10484752492917215No Hit
ATATTAGTGTTGACATAACTCACTACTAGGTCCCTCGATGCTTGATCTTC940.10484752492917215No Hit
GTGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTAT940.10484752492917215No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC940.10484752492917215No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG930.10373212572779798No Hit
ATGTCTCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG920.1026167265264238No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT910.10150132732504964No Hit
CATTTAAACCCTGACAAAACTAAAAGATGGGGTTACTCTTTAAATTTTAT910.10150132732504964No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA900.10038592812367546No Hit
ACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCG900.10038592812367546No Hit
GGCCAGGCCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC900.10038592812367546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)