FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002490110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177730
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9310.523828278849941No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA8030.45180892364823044No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7310.4112980363472683No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4740.266696674731334No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4720.2655713723063073No Hit
ATCTCCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCT4680.2633207674562539No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4560.2565689529060935No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG4510.2537556968435267No Hit
GAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAAT4130.2323749507680189No Hit
ATTCTATGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGGGCTAA3820.21493276318010468No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3710.20874359984245763No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC3660.20593034377989083No Hit
GCAAATAATTGCTTGCCTGAGTGCAGTATGATGAGGTGAACAATGCTCAG3590.2019917852922973No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3440.19355201710459685No Hit
GTAGAGTTTCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCGCGCT2990.16823271254149552No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT2980.16767006132898216No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC2850.16035559556630843No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2760.1552917346536882No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAG2600.14628931525347438No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA2580.14516401282844765No Hit
ATGATGGTGTTTTCCAATGAGGGTTAAAGACAGGTACAGTAGAAGAATAA2550.14347605919090756No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2510.1412254543408541No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2440.13728689585326057No Hit
GGGTCACTCTTTAAATTTTATGGGCTATAGCATTGGATGTTATGGGTCAT2430.1367242446407472No Hit
GGTAATTGCAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAGGT2420.13616159342823383No Hit
GTTCCGCGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGAGAGG2390.13447363979069374No Hit
TAATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAG2390.13447363979069374No Hit
CTCTAAGACTGTTTGTGTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2370.133348337365667No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC2320.1305350813031002No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT2310.12997243009058684No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT2290.1288471276655601No Hit
GGGTAAAGGGTCAGATATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA2180.12265796432791314No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC2160.1215326619028864No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT2120.11928205705283294No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2110.11871940584031958No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT2090.11759410341529286No Hit
ATATTAGTGTTGACATAACTCACTACTAGGTCCCTAGATGCTGGATCATC2090.11759410341529286No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAAT2090.11759410341529286No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACATCACGTAGCGCC2070.11646880099026613No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC2070.11646880099026613No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2020.11365554492769933No Hit
GTTTGCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG1990.11196759129015924No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTCACTCTTTAAATTTTAT1980.11140494007764587No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT1970.1108422888651325No Hit
ATACATAGAGGTTCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCG1960.11027963765261914No Hit
CAGACGGGCAACATACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAG1960.11027963765261914No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1950.10971698644010577No Hit
CTCAAAGGTGGAGTCAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGT1950.10971698644010577No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1890.10634107916502561No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTCGTCAGGCTTTCACTTT1870.10521577673999888No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT1830.10296517188994542No Hit
ATTATTTGCTGGGGGGAACTAATGACTCTAGCTACCTGGGTGGGTGCTAA1830.10296517188994542No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT1810.10183986946491869No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT1810.10183986946491869No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC1810.10183986946491869No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAGCA1810.10183986946491869No Hit
CTGTTTGTGTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGC1800.10127721825240532No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA1790.10071456703989196No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT1790.10071456703989196No Hit
GCTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC1780.1001519158273786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)