FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177730
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9270.5215776739998875No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7340.41298598998480845No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7160.4028582681595678No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5020.2824509086817082No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4760.26782197715636075No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4560.2565689529060935No Hit
ATCTCCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCT4390.24700388229336634No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4140.23293760198053226No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3790.21324480954256456No Hit
GAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAAT3590.2019917852922973No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC3520.19805322680470377No Hit
ATTCTATGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGGGCTAA3290.18511224891689643No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3210.1806110392167895No Hit
GCAAATAATTGCTTGCCTGAGTGCAGTATGATGAGGTGAACAATGCTCAG3120.17554717830416924No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT2930.16485680526641533No Hit
GTAGAGTTTCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCGCGCT2870.16148089799133516No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA2700.151915827378608No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2480.13953750070331403No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT2350.13222303494064028No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2340.13166038372812694No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2340.13166038372812694No Hit
GGGTCACTCTTTAAATTTTATGGGCTATAGCATTGGATGTTATGGGTCAT2320.1305350813031002No Hit
ATATTAGTGTTGACATAACTCACTACTAGGTCCCTAGATGCTGGATCATC2300.12940977887807348No Hit
CTCTAAGACTGTTTGTGTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2270.12772182524053338No Hit
TAATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAG2240.12603387160299329No Hit
GTTCCGCGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGAGAGG2230.12547122039047995No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAG2190.1232206155404265No Hit
ATGATGGTGTTTTCCAATGAGGGTTAAAGACAGGTACAGTAGAAGAATAA2120.11928205705283294No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2100.11815675462780621No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT2090.11759410341529286No Hit
GTTTGCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG2040.11478084735272605No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT2030.11421819614021268No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTCACTCTTTAAATTTTAT1980.11140494007764587No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTCGTCAGGCTTTCACTTT1980.11140494007764587No Hit
ATACATAGAGGTTCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCG1950.10971698644010577No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC1950.10971698644010577No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT1910.10746638159005234No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCATTTCCTAAT1880.10577842795251224No Hit
GGTAATTGCAGGCCTCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAGGT1870.10521577673999888No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC1830.10296517188994542No Hit
CTCAAAGGTGGAGTCAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGT1800.10127721825240532No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC1800.10127721825240532No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT1800.10127721825240532No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT1790.10071456703989196No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1790.10071456703989196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)