FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413428
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA23560.5698694815058487No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC15840.3831380554776164No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15330.3708021711156477No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15290.3698346507735325No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT14310.3461304023917103No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12900.31202531033214975No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11790.28517662083845313No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT11610.2808227792989348No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC11170.27018005553566765No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10960.26510057373956286No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG10860.26268177288427486No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10690.25856981143028535No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10670.25808605125922773No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10160.24575016689725898No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10160.24575016689725898No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9980.24139632535774064No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9900.23946128467351024No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9700.2346236829629343No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT9700.2346236829629343No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT9650.2334142825352903No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9610.23244676219317512No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9570.2314792418510599No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9250.22373907911413837No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC8730.21116131466664087No Hit
TACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAAC8720.21091943458111206No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8640.20898439389688167No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8390.2029373917586617No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8130.19664850953491297No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7970.19277842816645221No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7920.1915690277388082No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAAC7840.18963398705457782No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7660.18528014551505945No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7650.18503826542953067No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7630.18455450525847306No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG7360.1780237429491955No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7270.1758468221794363No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA7170.17342802132414833No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7050.17052546029780274No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6960.16834853952804357No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6950.16810665944251477No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6680.16157589713323722No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6510.15746393567924766No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6480.15673829542266127No Hit
GTACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6390.1545613746529021No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6380.1543194945673733No Hit
TCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG6290.1521425737976141No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6260.1514169335410277No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATACGCTGATGGCC6250.15117505345549892No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAGGCTGCTAGGAGTTCCGCA6210.1502075331133837No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6200.14996565302785492No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGG6180.1494818928567973No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6150.14875625260021091No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6050.14633745174492294No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6050.14633745174492294No Hit
GTACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6040.14609557165939413No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5840.14125796994881817No Hit
ACTCACCCCAACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAA5800.14029044960670298No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5720.13835540892247258No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5720.13835540892247258No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5650.13666224832377102No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5540.1340015673829542No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5510.13327592712636785No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5490.13279216695531024No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5450.13182464661319504No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5400.13061524618555106No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5390.13037336610002226No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5390.13037336610002226No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5350.12940584575790706No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5320.12868020550132067No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG5250.12698704490261908No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5210.1260195245605039No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5200.12577764447497508No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5170.1250520042183887No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5110.12360072370521591No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5110.12360072370521591No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5100.1233588436196871No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5100.1233588436196871No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5080.12287508344862949No Hit
GGATTAAAGACAGGTACAGAAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5020.12142380293545672No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4980.12045628259334151No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4950.11973064233675512No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4880.11803748173805355No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4880.11803748173805355No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4870.11779560165252476No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4850.11731184148146714No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGTAAATTGCCTGTAA4830.11682808131040956No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4830.11682808131040956No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4820.11658620122488075No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4780.11561868088276557No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4770.11537680079723676No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4720.11416740036959278No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4710.11392552028406397No Hit
GTCTATTTACAGGCAATTTACGAAAACATTGCTTGAGTTTTAGTACAATA4700.11368364019853516No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC4680.11319988002747759No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4670.11295799994194879No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4630.11199047959983359No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4610.11150671942877599No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGCTGCTGGCACT4600.11126483934324718No Hit
CGCATACGCTGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTC4580.1107810791721896No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT4560.110297319001132No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA4550.1100554389156032No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCAAATGCTCCCG4540.10981355883007439No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4470.10812039823137282No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4470.10812039823137282No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4450.10763663806031522No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4440.10739475797478641No Hit
TCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGTGAGGCA4420.10691099780372883No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGTATGCGTGGAACCTTTGTGGC4410.10666911771820002No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4350.10521783720502723No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4330.10473407703396964No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTAAAG4310.10425031686291204No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4300.10400843677738324No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTA4290.10376655669185446No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4290.10376655669185446No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4250.10279903634973925No Hit
GGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGGAAGCAGAGGAC4220.10207339609315286No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4220.10207339609315286No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4210.10183151600762405No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4200.10158963592209526No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4190.10134775583656647No Hit
CGGTAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAACA4190.10134775583656647No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG4140.10013835540892248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)