FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413428
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19360.46827984558375335No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16270.3935388991553547No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15080.3647551689774277No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC12520.3028338670820554No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12190.29485182425960504No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT12060.2917073831477307No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT11610.2808227792989348No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG10950.2648586936540341No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10630.25711853091711256No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10320.2496202482657198No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10250.2479270876670182No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10210.246959567324903No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10040.24284760587091345No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT10040.24284760587091345No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9990.24163820544326944No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9720.2351074431339919No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT9450.22857668082471436No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9410.22760916048259913No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9220.22301343885755198No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9160.2215621583443792No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8860.21430575577851527No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8860.21430575577851527No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8100.19592286927832656No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7990.1932621883375098No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7970.19277842816645221No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC7690.18600578577164584No Hit
TACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAAC7680.18576390568611703No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7600.18382886500188667No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7510.1816519442321275No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7480.1809263039755411No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC7340.1775399827781379No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAAC7290.1763305823504939No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7250.17536306200837873No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7130.17246050098203317No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7120.17221862089650436No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7110.17197674081097555No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7060.17076734038333155No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7000.16931605987015877No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA6980.16883229969910118No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA6720.16254341747535242No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6690.16181777721876603No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6670.16133401704770842No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6640.16060837679112205No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG6480.15673829542266127No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6400.15480325473843087No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG6340.1533519742252581No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6280.1519006937120853No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6240.15093317336997011No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6040.14609557165939413No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATACGCTGATGGCC6010.14536993140280774No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5740.1388391690935302No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5720.13835540892247258No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5690.1376297686658862No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGG5590.13521096781059821No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5580.1349690877250694No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5550.13424344746848302No Hit
ACTCACCCCAACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAA5530.13375968729742543No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAGGCTGCTAGGAGTTCCGCA5510.13327592712636785No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5510.13327592712636785No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5480.13255028686978143No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5440.13158276652766623No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5360.12964772584343587No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5330.12892208558684945No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5320.12868020550132067No Hit
GTCTATTTACAGGCAATTTACGAAAACATTGCTTGAGTTTTAGTACAATA5310.12843832541579187No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG5310.12843832541579187No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5300.12819644533026306No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5250.12698704490261908No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5230.12650328473156147No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5140.12432636396180229No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGTATGCGTGGAACCTTTGTGGC5130.1240844838762735No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5070.12263320336310071No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5000.12094004276439912No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4960.11997252242228393No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4920.11900500208016875No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4900.11852124190911113No Hit
GGATTAAAGACAGGTACAGAAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4880.11803748173805355No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4870.11779560165252476No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4870.11779560165252476No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4860.11755372156699595No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4810.11634432113935196No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT4760.11513492071170796No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4760.11513492071170796No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4740.11465116054065036No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGTAAATTGCCTGTAA4710.11392552028406397No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4550.1100554389156032No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4550.1100554389156032No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4510.10908791857348801No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4490.1086041584024304No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4480.10836227831690162No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC4460.10787851814584401No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4460.10787851814584401No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4440.10739475797478641No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4430.10715287788925763No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGTAAATTGCCTGTAAATAGA4410.10666911771820002No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4410.10666911771820002No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4390.10618535754714244No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4370.10570159737608484No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4360.10545971729055603No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4360.10545971729055603No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4350.10521783720502723No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGCTGCTGGCACT4340.10497595711949845No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4340.10497595711949845No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTG4330.10473407703396964No Hit
GTACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4320.10449219694844084No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTAAAG4310.10425031686291204No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4290.10376655669185446No Hit
GCGTATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAAC4280.10352467660632565No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4280.10352467660632565No Hit
TCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGTGAGGCA4240.10255715626421047No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4210.10183151600762405No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4200.10158963592209526No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4190.10134775583656647No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4170.10086399566550887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)