FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376686
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA23580.6259855688823052No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC21390.5678469600675364No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT17510.4648433974185396No Hit
ATATAGGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT17280.4587375161274908No Hit
TCTTTGGAGTTACTCTCGTTTTTGCCTAATGACTTCTTTCCTTCCGCCAG17180.4560827851313827No Hit
GGGTCTTTCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTC15430.4096249926994897No Hit
GTTTAGCTTTCAGCTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA15370.4080321541018249No Hit
ATCAAGGTATGTTGCGCGTTTGTCATCTAATTCCAGGATCAACAACAACC14920.39608586461933815No Hit
GTACGGGACCGTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG14920.39608586461933815No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGATGACAAACGCGCAAC14580.38705977923257034No Hit
GGAAAACCCCGCCTGTAACACGAGCAGGGGTCCTAGGAATCCTGATGTGA14560.38652883303334873No Hit
GACTAAGGCCCACTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGA14510.38520146753529466No Hit
GAATACTAACATTGGGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA12090.3209569774294771No Hit
TTGCACACCTCACCATACTGCACTCAGGCAAGCCATTGTCTGCTGGGGGG11490.3050285914528281No Hit
GTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAAC11220.29786081776333606No Hit
CTCCTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT10920.28989662477501155No Hit
CTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCA10530.27954317389018973No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAGACCCC10360.2750301311968058No Hit
AAGCCAAACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA9500.2521994446302756No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9120.24211146684506457No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9000.2389257896497348No Hit
ATTAGTCACAGCGGTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCC8560.22724497326685889No Hit
AAGTCTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCCAGTGGG8370.22220098437425334No Hit
GTTAGCATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC8370.22220098437425334No Hit
ACTAGATCCCTGGATGCTGGATCTTGCAAATTATTACCCACCCAGGTAGC8230.21848436097970195No Hit
GTCTAGACTCGTGGTGGACTTCTCACAATTTTCTAGGGGGATCACCCGTG8030.2131748989874856No Hit
GAATCTTAGGAAAGGAAGGAGTTTGCCATTCAGGATTAAAGATAGGTACT7760.2060071252979936No Hit
TCTGTTGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCCGTCAG7730.2052107059991611No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7710.20467975979993946No Hit
CAAGAAAGACCCCGCCTGTAACACGAGCAGGGGTCCTAGGAATCCTGATG7590.2014940826046097No Hit
GTTTTCCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTCGT7540.20016671710655556No Hit
TACCCTGATAATCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG7420.19698103991122579No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA7310.19406083581550682No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG7270.19299894341706356No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATATTAGGAAAGGAAGGAGTTT7270.19299894341706356No Hit
GACTGAGGCCCACTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGA7040.18689306212601478No Hit
TCTTTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCCGTCAG7020.18636211592679314No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6740.17892886913769027No Hit
CAGACAGATTCGTCCCCATGCCTTTGCGAGGTTTTGATGACCAACCTCCC6740.17892886913769027No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATAGTTAAGTA6610.17547771884274965No Hit
GAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCT6520.17308846094625233No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA6490.17229204164741987No Hit
CTCTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6410.17016825685053336No Hit
GCTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA6390.1696373106513117No Hit
GCCTTAGAGTCTCCTGAGCATGGCTCACCTCACCATACTGCACTCAGGCA6360.16884089135247923No Hit
ACAAAGTCTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCCAGT6340.16830994515325762No Hit
GTACGGGGCCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6250.16592068725676026No Hit
GTTAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA6250.16592068725676026No Hit
GTACTGTAGAGGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6210.164858794858317No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6200.1645933217587062No Hit
AAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA6190.16432784865909536No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6190.16432784865909536No Hit
TAATTTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGATAATAAGG6170.16379690245987374No Hit
GTACTTAACTATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5860.15556723637193845No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5850.15530176327232761No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5830.15477081707310597No Hit
CTATATAGGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5780.1534434515750519No Hit
CTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA5730.1521160860769978No Hit
GCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTGCCATTTGTTCAGTG5620.14919588198127884No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5620.14919588198127884No Hit
ATCTTTCTGTTCCCAACCCGCTGGGATTCCTTCCCGATCATCAGTTGGAC5540.1470720971843923No Hit
GAATGCTAACATTGGGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA5530.14680662408478148No Hit
ATTCAGGATTAAAGATAGGTACTGTAGAGGAGTAAAGCCCAGTAAAGTTT5520.14654115098517068No Hit
ACAACAACCAGTACGGGACCGTGCAAAACCTGCACGACTCCTGCTCAAGG5520.14654115098517068No Hit
TACCCTGATAATCCAGAAGAACCAATAAGAAGATGAGGCATGGCAGCAGG5520.14654115098517068No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5490.14574473168633822No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC5420.14388641998906251No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5360.1422935813913976No Hit
TGGTAGACTTCTCACAATTTTCTAGGGGGATCACCCGTGTGTCTTGGCCA5250.13937337729567864No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5120.13592222700073803No Hit
GTAGAGGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5080.13486033460229477No Hit
ATTCAGACCCATGCCCTGAGCCTGAGGGTCCAACTGATGATCGGGAAGGA5070.13459486150268393No Hit
TGGCTCACCTCACCATACTGCACTCAGGCAAGCCATTGTCTGCTGGGGGG5060.13432938840307312No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTGACGT4970.13194013050657577No Hit
TATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATG4940.13114371120774332No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCAACAGCAACC4920.13061276500852168No Hit
GATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGGCA4920.13061276500852168No Hit
GTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCC4880.12955087261007842No Hit
GATCAAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGGCA4830.12822350711202435No Hit
CCAGACATTATTTACATACTCTTTGGAAGGCTGGTGTTCTATATAGGAGG4820.1279580340124135No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4790.12716161471358106No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4750.1260997223151378No Hit
GTCTCGTGGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGG4720.12530330301630538No Hit
GCATAGCAGCAGGATGAAGAGGAATATGATAAAATGCCGCCGACACATCC4700.12477235681708372No Hit
CCTAATATTCATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGT4680.12424141061786209No Hit
GTCCACCACGAGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAA4680.12424141061786209No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4670.12397593751825127No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT4620.12264857202019719No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4570.12132120652214311No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4570.12132120652214311No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGGCCCTTTATACC4550.12079026032292146No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT4480.11893194862564577No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4470.11866647552603496No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4430.11760458312759167No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4420.11733911002798088No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT4420.11733911002798088No Hit
CTGTAGAGGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGA4400.11680816382875923No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4380.11627721762953759No Hit
GTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA4380.11627721762953759No Hit
ACTCCAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTG4360.11574627143031598No Hit
TCTGTTGAGTTACTCTCGTTTTTGCCTAATGACTTCTTTCCTTCCGCCAG4340.11521532523109433No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT4330.11494985213148351No Hit
GCCTAGTCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4300.11415343283265107No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAATCACCCGTG4300.11415343283265107No Hit
GGATCATATTGTACAAAAGATCAAACATTGTTTTAGAAAACTTCCTGTTA4260.1130915404342078No Hit
CTCCGAAAGAGACCAAATAGTTAAGTACAGTCTCTCTTCCAAAAGTAAGG4250.11282606733459698No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4250.11282606733459698No Hit
AAGCTAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA4190.11123322873693207No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG4150.11017133633848882No Hit
ATCCCGAATGGCAAACTCCTTCCTTTCCTAATATTCATTTACAAGAGGAC4080.1083130246412131No Hit
CTTTAGGACATGGACATTGACACTTATAAAGAATATGGAGCTTCTGTTGA4070.10804755154160228No Hit
AGTCCACCACGAGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACA4040.10725113224276984No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA3980.10565829364510494No Hit
GGATCAAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGGC3980.10565829364510494No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT3970.10539282054549412No Hit
GGGTTTTCCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTC3910.10379998194782923No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT3860.10247261644977515No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA3820.10141072405133189No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA3820.10141072405133189No Hit
GCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATG3790.10061430475249943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)