FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376686
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA28470.7558019145919944No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC19660.521920113834865No Hit
ATATAGGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT16720.4438710225492851No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGATGACAAACGCGCAAC16210.43033189446913345No Hit
TCTTTGGAGTTACTCTCGTTTTTGCCTAATGACTTCTTTCCTTCCGCCAG15870.4213058090823657No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT15080.40033343421311113No Hit
GTACGGGACCGTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG14580.38705977923257034No Hit
GTTTAGCTTTCAGCTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA14130.37511348975008363No Hit
GGGTCTTTCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTC13720.36422909266604014No Hit
GGAAAACCCCGCCTGTAACACGAGCAGGGGTCCTAGGAATCCTGATGTGA13410.35599942657810485No Hit
GAATACTAACATTGGGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA12750.338478202003791No Hit
ATCAAGGTATGTTGCGCGTTTGTCATCTAATTCCAGGATCAACAACAACC12680.33661989030651523No Hit
GACTAAGGCCCACTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGA12620.33502705170885033No Hit
GTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAAC10820.28724189377890336No Hit
CTCCTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT10180.2702516154038111No Hit
TTGCACACCTCACCATACTGCACTCAGGCAAGCCATTGTCTGCTGGGGGG9370.248748294335335No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9340.24795187503650254No Hit
CTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCA8940.23733295105206992No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATAGTTAAGTA8920.23680200485284827No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAGACCCC8480.22512118846997237No Hit
GTTAGCATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC8470.22485571537036153No Hit
AAGCCAAACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA8360.22193551127464256No Hit
GAATCTTAGGAAAGGAAGGAGTTTGCCATTCAGGATTAAAGATAGGTACT8250.21901530717892354No Hit
ATTAGTCACAGCGGTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCC8160.2166260492824262No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATATTAGGAAAGGAAGGAGTTT8100.21503321068476133No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8030.2131748989874856No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7960.21131658729020988No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7850.20839638319449091No Hit
ACTAGATCCCTGGATGCTGGATCTTGCAAATTATTACCCACCCAGGTAGC7700.20441428670032866No Hit
AAGTCTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCCAGTGGG7340.19485725511433927No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC7320.19432630891511762No Hit
GTCTAGACTCGTGGTGGACTTCTCACAATTTTCTAGGGGGATCACCCGTG7210.19140610481939865No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG7130.18928232002251213No Hit
TCTGTTGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCCGTCAG7120.1890168469229013No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA7090.18822042762406885No Hit
GTTAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA6900.18317643873146333No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6790.18025623463574436No Hit
TAATTTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGATAATAAGG6760.1794598153369119No Hit
GACTGAGGCCCACTCCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGA6680.1773360305400254No Hit
GTACTGTAGAGGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6590.17494677264352804No Hit
TACCCTGATAATCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG6560.17415035334469559No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA6540.17361940714547394No Hit
GAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCT6510.1728229878466415No Hit
GCCTTAGAGTCTCCTGAGCATGGCTCACCTCACCATACTGCACTCAGGCA6430.17069920304975497No Hit
TACCCTGATAATCCAGAAGAACCAATAAGAAGATGAGGCATGGCAGCAGG6420.17043372995014414No Hit
TCTTTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCCGTCAG6410.17016825685053336No Hit
GTTTTCCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTCGT6350.16857541825286845No Hit
CTCTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6310.16751352585442517No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6220.16512426795792784No Hit
CAGACAGATTCGTCCCCATGCCTTTGCGAGGTTTTGATGACCAACCTCCC6010.15954933286610068No Hit
CTATATAGGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5990.15901838666687906No Hit
ACAAAGTCTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCCAGT5980.15875291356726823No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5970.15848744046765742No Hit
ATTCAGGATTAAAGATAGGTACTGTAGAGGAGTAAAGCCCAGTAAAGTTT5940.15769102116882497No Hit
CAAGAAAGACCCCGCCTGTAACACGAGCAGGGGTCCTAGGAATCCTGATG5750.15264703227621945No Hit
GTACTTAACTATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5700.15131966677816536No Hit
GTAGAGGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5680.15078872057894374No Hit
GTACGGGGCCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG5620.14919588198127884No Hit
GCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTGCCATTTGTTCAGTG5580.14813398958283555No Hit
ATCTTTCTGTTCCCAACCCGCTGGGATTCCTTCCCGATCATCAGTTGGAC5540.1470720971843923No Hit
AAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA5500.14601020478594906No Hit
CTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA5470.14521378548711658No Hit
GAATGCTAACATTGGGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA5470.14521378548711658No Hit
GCTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA5410.14362094688945168No Hit
ACAACAACCAGTACGGGACCGTGCAAAACCTGCACGACTCCTGCTCAAGG5390.14309000069023003No Hit
GTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCC5250.13937337729567864No Hit
CTCCGAAAGAGACCAAATAGTTAAGTACAGTCTCTCTTCCAAAAGTAAGG5240.1391079041960678No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5190.13778053869801374No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT5140.13645317319995964No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC4990.13247107670579739No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC4940.13114371120774332No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCAACAGCAACC4920.13061276500852168No Hit
GGATCATATTGTACAAAAGATCAAACATTGTTTTAGAAAACTTCCTGTTA4910.13034729190891087No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4900.13008181880930006No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4890.12981634570968925No Hit
CTGTAGAGGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGA4850.12875445331124596No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGGCCCTTTATACC4840.12848898021163516No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTGACGT4820.1279580340124135No Hit
GCCTAGTCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4740.125834249215527No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4740.125834249215527No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4720.12530330301630538No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4710.12503782991669454No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT4680.12424141061786209No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4650.12344499131902965No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4590.12185215272136475No Hit
GTCTCGTGGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGG4580.12158667962175393No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4560.12105573342253229No Hit
ATTCAGACCCATGCCCTGAGCCTGAGGGTCCAACTGATGATCGGGAAGGA4510.1197283679244782No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4440.1178700562272025No Hit
GGATCAAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGGC4350.11548079833070515No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT4340.11521532523109433No Hit
GCATAGCAGCAGGATGAAGAGGAATATGATAAAATGCCGCCGACACATCC4320.1146843790318727No Hit
GTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA4320.1146843790318727No Hit
CCAGACATTATTTACATACTCTTTGGAAGGCTGGTGTTCTATATAGGAGG4300.11415343283265107No Hit
ACTCCAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTG4280.11362248663342944No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT4260.1130915404342078No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4260.1130915404342078No Hit
GATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGGCA4260.1130915404342078No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4230.11229512113537536No Hit
CCTAATATTCATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGT4220.11202964803576454No Hit
TGGTAGACTTCTCACAATTTTCTAGGGGGATCACCCGTGTGTCTTGGCCA4170.11070228253771046No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAATCACCCGTG4160.11043680943809964No Hit
TGGCTCACCTCACCATACTGCACTCAGGCAAGCCATTGTCTGCTGGGGGG4150.11017133633848882No Hit
GATCAAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGGCA4140.109905863238878No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4100.10884397084043473No Hit
GTCCACCACGAGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAA4070.10804755154160228No Hit
TCTGTTGAGTTACTCTCGTTTTTGCCTAATGACTTCTTTCCTTCCGCCAG3970.10539282054549412No Hit
ATCCCGAATGGCAAACTCCTTCCTTTCCTAATATTCATTTACAAGAGGAC3900.10353450884821841No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3890.10326903574860759No Hit
CTCCTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATTTCTGGT3890.10326903574860759No Hit
TATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATG3870.10273808954938597No Hit
AAGCTAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA3870.10273808954938597No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA3870.10273808954938597No Hit
CCTCACCATACTGCACTCAGGCAAGCCATTGTCTGCTGGGGGGAATTGAT3860.10247261644977515No Hit
CTTTAGGACATGGACATTGACACTTATAAAGAATATGGAGCTTCTGTTGA3860.10247261644977515No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG3850.10220714335016433No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG3840.1019416702505535No Hit
GGATTAAAGATAGGTACTGTAGAGGAGTAAAGCCCAGTAAAGTTTCCCAC3820.10141072405133189No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT3800.10087977785211025No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3800.10087977785211025No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT3790.10061430475249943No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC3790.10061430475249943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)