FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391045
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC19300.49354933575419707No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19030.48664475955452696No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18670.4774386579549668No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14200.3631295630937616No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13540.3462517101612347No Hit
CTCTAGGACCGTTTCTCTTCCAAACGTGAGACAAGAAATGTGAAACCACA10950.280018923653288No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC10350.26467542098735436No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10260.2623738955874644No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10040.25674794460995537No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9170.23449986574435167No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG9120.23322124052219054No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCACTACCAAACGCCAGTC9100.23270979043332607No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8660.2214578884783081No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT8540.2183891879451214No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8420.21532048741193469No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7890.2017670600570267No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7800.19946553465713662No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC7770.19869835952383996No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7750.19818690943497552No Hit
CATTTACACCAAGATATTATTAAAAAATGTGAACAATTTGTAGGCCCACT7620.19486248385735658No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAGGA7350.18795790765768647No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT7330.187446457568822No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7160.18309913181347415No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6890.17619455561380404No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6880.17593883056937182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6800.173893030213914No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6780.17338158012504956No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6680.17082432968072728No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6560.16775562914754055No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6550.16749990410310833No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6360.16264112825889604No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6320.16161822808116713No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6300.1611067779923027No Hit
GATACATAGAGGTCCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6270.160339602859006No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6240.15957242772570931No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6190.1582938025035482No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6180.15803807745911597No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA5980.15292357657047143No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5880.15036632612614917No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5820.1488319758595558No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5750.14704190054853022No Hit
CAATAGGCCTGTTGATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5690.14550755028193685No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5690.14550755028193685No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACCGAGT5660.1447403751486402No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTTT5640.14422892505977572No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5630.1439732000153435No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5530.14141594957102124No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5520.14116022452658902No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5500.14064877443772456No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5480.14013732434886012No Hit
GTCCCGTGCTGGTGGTCGAAGATCCTGGAATCAGAGGACAAACGGGCAAC5470.1398815993044279No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCA5460.13962587425999567No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5410.13834724903783452No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCTTCGACCACC5400.1380915239934023No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG5330.1363014486823767No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5320.13604572363794448No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5300.13553427354908001No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTGACCAATTTTCTT5280.13502282346021557No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5150.13169839788259663No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAAA5080.12990832257157103No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5060.1293968724827066No Hit
CATACTGCTGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC5050.12914114743827437No Hit
ACATAGAGGTCCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4950.12658389699395212No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4940.12632817194951987No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4930.12607244690508765No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4850.12402664654962985No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4770.12198084619417203No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGGACCTCTATG4760.12172512114973981No Hit
GAATCTTTCCACCAGCAATCCTTTGGGATTCTTTCCCGACCACCAGTTGG4730.12095794601644312No Hit
CCTTATGAGTCCAAGGAACACTAACATTGAGATTCCCGAGATTGAGATCT4710.12044649592757868No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4660.11916787070541753No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAC4650.1189121456609853No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4630.11840069557212086No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4590.11737779539439194No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4590.11737779539439194No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACCGCATCTTGAGTCCCTTTTTACC4500.1150762699945019No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4490.11482054495006969No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4480.11456481990563747No Hit
ATGTATATTTGCGGGAGAGAACAACCGAGTTATCCGTCCCGAGAATGTTT4450.11379764477234078No Hit
GTTTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4360.11149611937245074No Hit
TCCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4340.1109846692835863No Hit
ATACTGCACTCAGGCAAGCAATTATTTGCTGGGGGGAACTAATGACTCTA4300.1099617691058574No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAACACTAAC4280.10945031901699293No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATC4230.1081716937948318No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT4170.10663734352823843No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGTTCCAG4110.10510299326164509No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4090.10459154317278062No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4090.10459154317278062No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4080.1043358181283484No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4080.1043358181283484No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4080.1043358181283484No Hit
CCATATTCTTGGGAACAAGAGATACAGCATGGGGCAGAATCTTTCCACCA4070.10408009308391618No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4040.10331291795061949No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4020.10280146786175505No Hit
TCACCATACTGCACTCAGGCAAGCAATTATTTGCTGGGGGGAACTAATGA4010.10254574281732282No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4000.10229001777289058No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC3970.10152284263959391No Hit
GTCCAAGGAACACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3960.10126711759516169No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3940.10075566750629722No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3930.100499942461865No Hit
GGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCGTTTGGTAGTGGC3930.100499942461865No Hit
GCATAGCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGC3930.100499942461865No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCTATGCAGTGGAACTCCA3920.10024421741743278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)