FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355536
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA21470.6038769632329778No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT18380.5169659331263219No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC15780.44383691103010664No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14060.3954592502587642No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12170.34230007650420774No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11940.3358309707033887No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10230.28773457540164704No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10230.28773457540164704No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9030.2539827190495477No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8720.24526348949192206No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8440.23738805634309887No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA8370.23541919805589306No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTAAACAATACC8360.23513793258629223No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8120.22838756131587237No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8090.2275437649070699No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA7720.21713694253183924No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7490.2106678367310202No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATA7410.20841771297421358No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7320.20588632374780613No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7070.1988546870077854No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGAAACTGGATCTTC6950.1954795013725755No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6900.19407317402457136No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6880.1935106430853697No Hit
ATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGAAGAGGCAGGTC6450.1814162278925341No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT6320.17775977678772334No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6260.17607218397011837No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6220.17494712209171506No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6220.17494712209171506No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6210.1746658566221142No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6040.16988434363890012No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5950.1673529544124927No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGGTCCCGTG5950.1673529544124927No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5870.16510283065568607No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5800.16313397236848026No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5770.1622901759596778No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5750.16172764502047612No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5680.15975878673327032No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5680.15975878673327032No Hit
GGGTAAAGGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA5500.15469600828045543No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5430.15272714999324963No Hit
GTTCATATCACCATACTGCAATCAGGCAAGCAGTTCTTTGCTGGGGGGAA5340.15019576076684218No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT5290.14878943341883805No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5270.14822690247963638No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5210.1465393096620314No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA5180.1456955132532289No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT5140.1445704513748256No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5120.14400792043562397No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG5050.14203906214841816No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAATACG4960.13950767292201072No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT4920.1383826110436074No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4890.1375388146348049No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4880.13725754916520408No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4870.13697628369560325No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4860.13669501822600244No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4850.1364137527564016No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCAGCCCAAAAGGCCTCCGT4810.13528869087799827No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAGTT4780.13444489446919583No Hit
GCTTTAAAGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4770.13416362899959497No Hit
GCACGGGACCATGCCGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4740.1333198325907925No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4740.1333198325907925No Hit
CCCCTATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGAAGAGGC4700.13219477071238916No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGGTCCC4670.1313509743035867No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4650.13078844336438505No Hit
ACCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4650.13078844336438505No Hit
ATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC4600.1293821160163809No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGCCTCT4600.1293821160163809No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4590.12910085054678008No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4590.12910085054678008No Hit
CATACCGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4580.12881958507717925No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4550.12797578866837678No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4550.12797578866837678No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC4520.12713199225957428No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4470.12572566491157014No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4420.124319337563566No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCATATCACCA4410.12403807209396517No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4390.1234755411547635No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4330.12178794833715854No Hit
AAAGTCCTCCGTGTGGTGGGGTGAATCCCAGCCCGAATGCTCCAGCTCCT4250.11953782458035192No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4200.11813149723234777No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC4200.11813149723234777No Hit
GGGTTACTCTTTACATTTCATGGGTTATGTCATTGGAGGTTATGGCTCAT4180.11756896629314612No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4140.11644390441474281No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4130.11616263894514199No Hit
GGAAAGTACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT4120.11588137347554116No Hit
AACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGC4100.1153188425363395No Hit
TAACATAACTGACTACTAGGTCCCTGGAAACTGGATCTTCCAAATTACCA4100.1153188425363395No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCGGCTGCGAGCAAAACA4080.11475631159713784No Hit
GGACCATGCCGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC4060.11419378065793619No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4020.11306871877953288No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT4000.11250618784033123No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3980.11194365690112956No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3910.10997479861392376No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT3900.10969353314432295No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3880.10913100220512129No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC3870.10884973673552045No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT3860.10856847126591962No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3850.1082872057963188No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC3850.1082872057963188No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAATCTATTCTCCTCC3840.10800594032671795No Hit
ACATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCG3820.1074434093875163No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3790.10659961297871383No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3790.10659961297871383No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3760.10575581656991134No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3730.10491202016110887No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3730.10491202016110887No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3710.10434948922190722No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3700.10406822375230637No Hit
GATATGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATATAGAGCTGAG3690.10378695828270555No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3680.10350569281310472No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATC3660.10294316187390307No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3660.10294316187390307No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG3660.10294316187390307No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3650.10266189640430225No Hit
CTCTTTACATTTCATGGGTTATGTCATTGGAGGTTATGGCTCATTGCCGC3640.10238063093470141No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT3640.10238063093470141No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3640.10238063093470141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)