FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355536
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT20510.5768754781512982No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA18810.5290603483191576No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC15970.4491809549525224No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13570.38167724224832367No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12330.34680032401782096No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG10530.2961725394896719No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9860.27732775302641643No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9830.276483956617614No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8980.25257639170154356No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8270.2326065433598848No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8190.23035641960307815No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8180.23007515413347732No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8110.22810629584627154No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7890.22191845551505332No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA7710.21685567706223843No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7700.2165744115926376No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA7430.20898024391341521No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7420.20869897844381444No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG7260.20419873093020113No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT7020.19744835965978128No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGAAACTGGATCTTC6900.19407317402457136No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATA6840.1923855812069664No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6800.19126051932856308No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6680.18788533369335314No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6540.18394761711894153No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6520.18338508617973986No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6230.17522838756131587No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6160.1732595292741101No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTAAACAATACC5850.1645402997164844No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT5680.15975878673327032No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5640.15863372485486701No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5530.1555398046892579No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA5470.15385221187165296No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5460.1535709464020521No Hit
GGGTAAAGGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA5390.15160208811484632No Hit
GCTTTAAAGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5390.15160208811484632No Hit
CCCCTATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGAAGAGGC5280.1485081679492372No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAATACG5260.14794563701003557No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5260.14794563701003557No Hit
ATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGAAGAGGCAGGTC5230.14710184060123307No Hit
GTTCATATCACCATACTGCAATCAGGCAAGCAGTTCTTTGCTGGGGGGAA5210.1465393096620314No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5210.1465393096620314No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG5160.14513298231402727No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5040.14175779667881733No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5020.1411952657396157No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5000.14063273480041402No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4980.14007020386121236No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4970.13978893839161152No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4930.13866387651320822No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4910.13810134557400655No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4910.13810134557400655No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGGTCCCGTG4910.13810134557400655No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4870.13697628369560325No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4860.13669501822600244No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4840.13613248728680077No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4810.13528869087799827No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4770.13416362899959497No Hit
ATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC4760.13388236352999414No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4750.13360109806039333No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT4730.13303856712119166No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4710.13247603618199003No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGGTCCC4650.13078844336438505No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCAGCCCAAAAGGCCTCCGT4620.12994464695558255No Hit
GCACGGGACCATGCCGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4600.1293821160163809No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4570.12853831960757842No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4500.1265694613203726No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4480.12600693038117097No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC4460.1254443994419693No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4410.12403807209396517No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCATATCACCA4360.12263174474596103No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4350.1223504792763602No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGCCTCT4290.12066288645875524No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4280.12038162098915439No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4270.12010035551955357No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4260.11981909004995274No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4260.11981909004995274No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT4220.11869402817154943No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4210.11841276270194862No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAGTT4180.11756896629314612No Hit
ACCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4170.1172877008235453No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4150.11672516988434364No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4140.11644390441474281No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4090.11503757706673866No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4080.11475631159713784No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4070.114475046127537No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT4040.11363124971873453No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4040.11363124971873453No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4030.1133499842491337No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT3990.11222492237073038No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATC3920.1102560640835246No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3910.10997479861392376No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC3890.1094122676747221No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT3870.10884973673552045No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3840.10800594032671795No Hit
CAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC3800.10688087844831465No Hit
GGACCATGCCGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC3800.10688087844831465No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3800.10688087844831465No Hit
GGGTTACTCTTTACATTTCATGGGTTATGTCATTGGAGGTTATGGCTCAT3780.106318347509113No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC3750.10547455110031052No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC3720.10463075469150802No Hit
CATACCGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3700.10406822375230637No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3700.10406822375230637No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3660.10294316187390307No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3650.10266189640430225No Hit
ATCCCAGAGTGAGAGGCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGA3580.10069303811709644No Hit
TATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCG3580.10069303811709644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)