FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239603
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12480.5208615918832402No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC8330.3476584183002717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7760.32386906674791216No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6790.2833854334044231No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6770.2825507193148667No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6530.27253415024018895No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6200.25876136776250713No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC6040.25208365504605534No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA5940.24791008459827296No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5500.22954637462803054No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGCAA5390.22495544713546992No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC5350.223286018956357No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5180.21619094919512694No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5110.21326944988167926No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4930.205757023075671No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC4900.20450495194133628No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4700.19615781104577154No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4570.19073216946365448No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC4260.17779410107552912No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4240.17695938698597263No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4160.17362053062774674No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4050.1690296031351861No Hit
GTTATTAGACGACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG3980.16610810382173846No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3820.15943039110528667No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3740.15609153474706075No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT3730.15567417770228253No Hit
GTGTACACTTCGCTTCACCTCTGCACGTCGCATGGAGACCACCGTGAACG3710.15483946361272605No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC3700.1544221065679478No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGTCTGATGTGAT3510.1464923227171613No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3510.1464923227171613No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3450.14398818044849188No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC3440.14357082340371363No Hit
GTCTAACAATAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT3390.14148403817982247No Hit
GACTTAATGACTCTAGCTACCTGGGTGGGTGGTAATTTGGAAGATCCAAT3370.14064932409026598No Hit
ATTGATGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA3370.14064932409026598No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3330.13897989591115303No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT3330.13897989591115303No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3290.1373104677320401No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT3280.13689311068726184No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCACTGTTCACCTCACCATACTG3260.13605839659770536No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC3250.13564103955292714No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC3240.1352236825081489No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3230.13480632546337065No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG3220.13438896841859244No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT3200.13355425432903595No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3180.1327195402394795No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT3160.131884826149923No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3140.1310501120603665No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3120.13021539797081005No Hit
AGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAA3120.13021539797081005No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG3110.1297980409260318No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3110.1297980409260318No Hit
AAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTA3090.12896332683647532No Hit
CTCTAGGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3040.12687654161258416No Hit
TACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3040.12687654161258416No Hit
CAAGAAAAACCCCGCCTGTAACACGAGCAGGGGTCCTAGGAATCCTGATG3020.12604182752302767No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2990.12478975638869295No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC2950.12312032820958002No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2920.12186825707524532No Hit
GCATAGGAGCCAGAAAGGTTCCACGCACGCGCTGATGGCCCATGACCAAG2900.12103354298568883No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2860.11936411480657587No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG2850.11894675776179765No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC2820.11769468662746292No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2810.11727732958268469No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2800.11685997253790645No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT2790.1164426154931282No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA2760.1151905443587935No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2740.11435583026923703No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT2730.11393847322445878No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC2730.11393847322445878No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG2730.11393847322445878No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT2730.11393847322445878No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT2720.11352111617968054No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT2680.11185168800056762No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT2680.11185168800056762No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAATCTCCTGA2660.11101697391101113No Hit
GTATGGATCGGCATAGGAGCCAGAAAGGTTCCACGCACGCGCTGATGGCC2650.1105996168662329No Hit
GTCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA2650.1105996168662329No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2650.1105996168662329No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG2640.11018225982145466No Hit
GGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACA2640.11018225982145466No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2620.10934754573189817No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC2610.10893018868711996No Hit
CATGTATTCAGTCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC2600.10851283164234171No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2570.107260760508007No Hit
GTGTACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA2550.1064260464184505No Hit
CATTAGTTCCCCCCAGCACAGAATTGCTTGCCTGAGAGCAGTATGGTGAG2530.10559133232889405No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA2520.1051739752841158No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2500.10433926119455933No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG2490.10392190414978109No Hit
TCTAACAATAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG2490.10392190414978109No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA2470.10308719006022463No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG2440.10183511892588992No Hit
AATAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTC2430.10141776188111168No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG2430.10141776188111168No Hit
GCCTTAGAATCTCCTGAGCACTGTTCACCTCACCATACTGCTCTCAGGCA2410.1005830477915552No Hit
GTAAAAGCCTGCTTAGACTGAATACATGCATACAAGGGCATTAAAGCAGG2400.10016569074677696No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT2400.10016569074677696No Hit
CATTTACATCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACT2400.10016569074677696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)