FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239603
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11940.4983243114652154No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7720.32219963856879924No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7570.3159392828971257No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6790.2833854334044231No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6470.27003000797151955No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6280.262100224120733No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6080.2537530832251683No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA5930.24749272755349472No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC5640.23538937325492584No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGCAA5110.21326944988167926No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5090.21243473579212283No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5070.21160002170256634No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4920.20533966603089276No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA4360.18196767152331147No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC4190.17487260176208144No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC4160.17362053062774674No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4080.17028167426952082No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3940.16443867564262551No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3820.15943039110528667No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3690.1540047495231696No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3690.1540047495231696No Hit
GTGTACACTTCGCTTCACCTCTGCACGTCGCATGGAGACCACCGTGAACG3560.1485791079410525No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT3510.1464923227171613No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3500.14607496567238307No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3490.14565760862760485No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3450.14398818044849188No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT3420.14273610931415717No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3380.14106668113504423No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3380.14106668113504423No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3370.14064932409026598No Hit
GACTTAATGACTCTAGCTACCTGGGTGGGTGGTAATTTGGAAGATCCAAT3340.13939725295593128No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3330.13897989591115303No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3290.1373104677320401No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT3270.1364757536424836No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG3260.13605839659770536No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3260.13605839659770536No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC3240.1352236825081489No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3230.13480632546337065No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC3210.1339716113738142No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA3200.13355425432903595No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3200.13355425432903595No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT3180.1327195402394795No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT3180.1327195402394795No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT3150.13146746910514476No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGTCTGATGTGAT3120.13021539797081005No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC3100.12938068388125357No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3080.1285459697916971No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC3080.1285459697916971No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG3060.12771125570214062No Hit
GTTATTAGACGACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG3040.12687654161258416No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3040.12687654161258416No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCACTGTTCACCTCACCATACTG3000.12520711343347118No Hit
TACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT2990.12478975638869295No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT2980.12437239934391472No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2980.12437239934391472No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC2930.12228561412002353No Hit
GTCTAACAATAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT2920.12186825707524532No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG2870.11978147185135411No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC2860.11936411480657587No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC2840.11852940071701941No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC2840.11852940071701941No Hit
GTATGGATCGGCATAGGAGCCAGAAAGGTTCCACGCACGCGCTGATGGCC2820.11769468662746292No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2800.11685997253790645No Hit
GTTTTCCTTGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTCGT2770.11560790140357174No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAATCTCCTGA2750.11477318731401527No Hit
CTCTAGGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2740.11435583026923703No Hit
GCATAGGAGCCAGAAAGGTTCCACGCACGCGCTGATGGCCCATGACCAAG2740.11435583026923703No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG2710.11310375913490232No Hit
CAAGAAAAACCCCGCCTGTAACACGAGCAGGGGTCCTAGGAATCCTGATG2700.11268640209012408No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2700.11268640209012408No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC2680.11185168800056762No Hit
CAAATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC2590.10809547459756347No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2540.10600868937367229No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA2510.10475661823933757No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2510.10475661823933757No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC2450.10225247597066814No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA2430.10141776188111168No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG2430.10141776188111168No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG2430.10141776188111168No Hit
CATGTATTCAGTCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC2400.10016569074677696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)