FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811137
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82541.0175839593065044No Hit
CTCTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT33960.4186715684280214No Hit
GGATAATAGGGTTTGATTCCTTTATCCAAGGGCAAGTATTTGGTAACTTT29550.364303440725796No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTCAA22770.2807170675237352No Hit
CTCCAAAAGACACTAAATATTCAAGAACAATTTCTCTTCCAAAAGTAAGA20970.2585259949922146No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT20350.25088240334246864No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTGCCTTAATTTCAT18450.22745849344808583No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA17980.22166415784263324No Hit
ATGTTAATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCAT17400.21451370113803217No Hit
GCTTACACCTATCAACAATATCTTCCTGCAAATGAATGTCAGGAAAAGAA17350.21389728245660103No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC15720.1938020334419463No Hit
GGGACTTAGTAGTCGGCTATGTTAATGTTAATATGGGCCTAAAACTCAGA14720.18147365981332378No Hit
CCCTAACAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTAGA14640.18048738992303398No Hit
GTTTACAGGAAGTTTCCTAAAACAGAGTTTGATTTTTTGTTTAATATGTT14370.17715872904330587No Hit
GGTGTAAGCAATTCGTGGGACCCCTAACAGTAAATGAAATCAGGAGACTA14040.17309036574586045No Hit
CTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCT14000.17259723080071554No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG13690.16877543497584255No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA13150.1621181132163864No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13040.1607619921172379No Hit
CCTTTATCCAAGGGCAAGTATTTGGTAACTTTAGGAAAAAATCTAGCAGG12850.15841960112779963No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT12610.15546079145693023No Hit
GTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAATTCGTTGACATACT12250.1510225769506261No Hit
CAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTTT12120.14941988837890516No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12000.14794048354347047No Hit
ACTTACCACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11970.1475706323346118No Hit
ACCAAATACTTGCCCTTGGATAAAGGAATCAAACCCTATTATCCCGAGCA11950.14732406486203933No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACAGAGTTTGATTTTTTGT11560.14251599914687654No Hit
CTCATAAGGTGGGAAACTTTATGGGGCTTTATTCTTCTACGGTACCTAAC11330.13968047321229338No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA11100.13684494727771018No Hit
ATATAAGAGAGAGACCACACATAGCGCCTCATTTTGCGGGTCACCATATT10900.13437927255198567No Hit
TGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCATATTTCCT10850.13376285387055453No Hit
ATGTGTGGTCTCTCTCTTATATAAGATACCCGCCTTCCAAAGAGTATGTA10640.1311738954085438No Hit
GGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCATTACT10600.1306807604633989No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG10220.12599597848452235No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTACTGACTTCTTTCCTTCTATTCG10130.12488642485794632No Hit
ATCCAAGGGCAAGTATTTGGTAACTTTAGGAAAAAATCTAGCAGGCATAA10050.12390015496765651No Hit
GTGGTAAGTATGCCCTGAGCATGAGGGCTCCACCCCAACAGGCCCCCATG9770.1204482103516422No Hit
CCTATCAACAATATCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT9740.12007835914278353No Hit
CCAAAAGACACTAAATATTCAAGAACAATTTCTCTTCCAAAAGTAAGACA9630.11872223804363505No Hit
GCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTGCCACAG9550.11773596815334525No Hit
GTTGATAGGTGTAAGCAATTCGTGGGACCCCTAACAGTAAATGAAATCAG9490.11699626573562788No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA9420.11613327958162431No Hit
GTATTTGGTAACTTTAGGAAAAAATCTAGCAGGCATAATTAATTTTAGTC9380.11564014463647941No Hit
GGGAATTAGTAGTCGGCTATGTTAATGTTAATATGGGCCTAAAACTCAGA9270.11428402353733094No Hit
ACCCCTAACAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTA9170.11305118617446867No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTATGGGGCTTTATTCTTCTACGG9020.11120193013017529No Hit
ATATTGTTGATAGGTGTAAGCAATTCGTGGGACCCCTAACAGTAAATGAA8870.10935267408588192No Hit
TCCTAAAACAGAGTTTGATTTTTTGTTTAATATGTTCCTGTGGCAAGGAA8840.10898282287702325No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAACCCCAGAAGACCCACAATTCG8720.10750341804158854No Hit
CTATTGTGGTTTCATATTTCCTGTCTTACTTTTGGAAGAGAAATTGTTCT8670.10688699936015741No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA8620.10627058067872629No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCATATTTCC8350.1029419197989982No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG8310.1024487848538533No Hit
CCTCCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAGAC8300.10232550111756707No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT8210.10121594749099103No Hit
AATTAAGGCAATACCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA8150.10047624507327368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)