FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346699
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11530.3325651357517616No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10300.2970876754764219No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9220.2659367347468553No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8380.2417082252905258No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACTTT8150.23507422865367364No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7930.22872866665320638No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7500.21632597728865674No Hit
ATATTAACGTTGACATAATTGACTACTAAATCCCTGGATGCTTGATCTTG7040.20305798401495245No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7000.20190424546941296No Hit
GACTCAAATTAATTATGCCTGCTAGGTTTTATCCCAAAGTTACTAAATAT6950.20046207228748858No Hit
AGTCTAGACTCTGTGGTATTGTTAGGATTTTTGTCAACAAGAAAAACCCC6920.19959676837833393No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6900.1990198991055642No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6490.1871940790137843No Hit
TTCAAGAACAGTGTCTCTCCCAAAAGTGAGACAGGAAATGTGAAACCACA6450.1860403404682448No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6380.18402129801355066No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6370.1837328633771658No Hit
ATATGTTCTTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA6020.17363765110369514No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5850.16873426228515223No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5600.16152339637553037No Hit
ATCCTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5600.16152339637553037No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5270.15200505337482947No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATCATAGGACAAACGGGCAAC5230.15085131482928996No Hit
ATTTTAGTCTCCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTCA5160.14883227237459584No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTGAGA4870.14046766791943444No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4840.1396023640102798No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4840.1396023640102798No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4790.13816019082835543No Hit
CTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4770.13758332155558567No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT4760.1372948869192008No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4690.1352758444645067No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4670.13469897519173693No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4640.1338336712825823No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA4280.12345002437272677No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4220.12171941655441751No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCAC4200.12114254728164778No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG4200.12114254728164778No Hit
CTCTTTGGAAGGCGGGAATCTTATATAAAAGAGAGTCCACACGTAGCGCC4130.11912350482695365No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC4130.11912350482695365No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACACTCCCCCATG4080.11768133164502927No Hit
TTAGAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGA4050.11681602773587463No Hit
GGTTTTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC3960.11422011600841074No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3920.11306637746287126No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3910.11277794282648637No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA3870.11162420428094688No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTCAAATTAATTATGCCTGCTAGG3870.11162420428094688No Hit
CTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3860.111335769644562No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTTCTCTTGTGCAAGACCTTGGG3850.11104733500817711No Hit
GGCTGAGACAGTATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCAC3760.10845142328071325No Hit
GTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC3750.10816298864432837No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3750.10816298864432837No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTGAGCAATTTG3720.10729768473517375No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3710.10700925009878887No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3710.10700925009878887No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3690.10643238082601911No Hit
GGGATTTAGTAGTCAATTATGTCAACGTTAATATGGGCCTAAAAATCAGA3680.10614394618963423No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT3660.10556707691686448No Hit
CCCATAGGTATTTTGCGGAAGCCCAAGATGATGGGATGGGAATACAGGTG3640.10499020764409472No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3590.10354803446217037No Hit
GTTCAGATACTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3570.1029711651894006No Hit
ATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGT3560.10268273055301573No Hit
CCTCCTGCATATAGACCAGCAAATGCCCCTATCCTATCAACACTTCCGGA3540.10210586128024599No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3540.10210586128024599No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT3510.10124055737109135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)