FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346699
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10510.3031448028405043No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8400.24228509456329556No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8400.24228509456329556No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7730.22295997392550887No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7440.2145953694703475No Hit
AGTCTAGACTCTGTGGTATTGTTAGGATTTTTGTCAACAAGAAAAACCCC7260.20940354601541972No Hit
ATATTAACGTTGACATAATTGACTACTAAATCCCTGGATGCTTGATCTTG7150.20623076501518608No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACTTT6750.19469337955979105No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6690.1929627717414818No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6660.19209746783232717No Hit
GACTCAAATTAATTATGCCTGCTAGGTTTTATCCCAAAGTTACTAAATAT6560.18921312146847843No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6450.1860403404682448No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6180.17825260528585316No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6020.17363765110369514No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5990.17277234719454052No Hit
TTCAAGAACAGTGTCTCTCCCAAAAGTGAGACAGGAAATGTGAAACCACA5910.1704648701034615No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5800.16729208910322788No Hit
ATCCTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5400.15575470364783284No Hit
CTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5340.1540240958295236No Hit
CTCTTTGGAAGGCGGGAATCTTATATAAAAGAGAGTCCACACGTAGCGCC5300.15287035728398407No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTGAGA5070.14623636064713194No Hit
ATATGTTCTTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA5000.14421731819243783No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5000.14421731819243783No Hit
ATTTTAGTCTCCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTCA4990.14392888355605293No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT4900.14133297182858906No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG4900.14133297182858906No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATCATAGGACAAACGGGCAAC4880.1407561025558193No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT4620.13325680200981255No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4600.1326799327370428No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4580.13210306346427306No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTCAAATTAATTATGCCTGCTAGG4510.13008402100957892No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4500.12979558637319402No Hit
GTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC4310.12431532828188141No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4200.12114254728164778No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA4190.1208541126452629No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4190.1208541126452629No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA4160.11998880873610826No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4070.11739289700864437No Hit
GTTCAGATACTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4030.1162391584631049No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3980.11479698528118051No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC3980.11479698528118051No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3920.11306637746287126No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTGAGCAATTTG3900.11248950819010149No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3880.11191263891733175No Hit
TTAGAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGA3870.11162420428094688No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT3800.10960516182625274No Hit
CCTCCTGCATATAGACCAGCAAATGCCCCTATCCTATCAACACTTCCGGA3780.109028292553483No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3750.10816298864432837No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3740.10787455400794348No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCAGCAAATGCC3670.10585551155324936No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT3670.10585551155324936No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACACTCCCCCATG3660.10556707691686448No Hit
GGTTTTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC3640.10499020764409472No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3620.10441333837132498No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3600.10383646909855523No Hit
CCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAA3570.1029711651894006No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3560.10268273055301573No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG3550.10239429591663085No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3550.10239429591663085No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3520.10152899200747623No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTC3510.10124055737109135No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCAC3480.10037525346193674No Hit
CTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3470.10008681882555184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)