FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569274
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCCGG36550.6420458338164048No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31590.5549173157389939No Hit
GGTCAGTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTT18190.3195297870621177No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG17060.2996799432259334No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA14810.2601559178883982No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13940.2448732947578846No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC13910.24434630775338412No Hit
GGATATTATCAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACG13900.24417064541855066No Hit
GTGTAAAAGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT12910.22678007427003516No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12860.22590176259586772No Hit
AAACTGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCC12500.21957791854186207No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12180.21395672382719041No Hit
AATAGGAAGTTTTCGAAAACATTGTTTGATTTTTTGTACAATATGTTCCT12170.21378106149235696No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11650.20464662008101547No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT11620.20411963307651498No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTGATAATATCCTCCTTC11430.2007820487146787No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCC11090.19480952933034004No Hit
GTGTGTGGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA11070.19445820466067307No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10950.1923502566426712No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG10780.18936399695050188No Hit
AATCTGGATTGTTTGAATTGGCTCCGAATGCAGGGTCCAACTGGTGACCG10720.18831002294150093No Hit
ATCCTAAACTGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10430.18321581523132974No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCGTTAAC10350.1818105165526618No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10190.17899991919532598No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA9990.17548667249865618No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTGTCTTCCAAACATAAGA9980.1753110101638227No Hit
GTATACATCTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAAC9590.16846017910531658No Hit
AGTCTGTACAACATCTTGAAGCCCTTTTTACCTCTATTACCAATTTTCTT9460.1661765687524812No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9390.1649469324086468No Hit
ATCCCGATTGGGACTTCAATCCCAACAAGGATCAGTGGCCAGCGGCAAAT8900.1563394780018058No Hit
ATTAATAGGAAGTTTTCGAAAACATTGTTTGATTTTTTGTACAATATGTT8870.15581249099730535No Hit
AACCGGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCC8750.15370454297930347No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8740.15352888064446998No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8590.15089394562196765No Hit
TAACCGGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATC8550.15019129628263367No Hit
GGTCCGGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTT8450.14843467293429877No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8310.14597540024662992No Hit
CTTTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG8270.14527275090729597No Hit
GTTTGGAAGTAATGATTAACTGTATGTTCCGGATAATAAGGTTTAATGCC8160.14334046522412755No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAGG7490.13157108879028379No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7410.13016579011161583No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA7340.12893615376778142No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCCCTTTTTACC7190.12630121874527908No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7060.1240176083924437No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6960.12226098504410882No Hit
GTACAACATCTTGAAGCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6900.12120701103510788No Hit
CTTCCCATTAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGG6890.12103134870027439No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG6890.12103134870027439No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATCTAAA6800.11945038768677299No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACA6760.11874773834743901No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6710.11786942667327156No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA6660.11699111499910411No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT6560.11523449165076922No Hit
ATAATATCCTCCTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6530.11470750464626876No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6430.11295088129793386No Hit
GTCAACGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACAT6370.11189690728893292No Hit
CCCTTAGATAAAGGCATTAAACCTTATTATCCGGAACATACAGTTAATCA6340.11136992028443246No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6290.110491608610265No Hit
GGGTAAAGGTCCAGATATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA6180.10855932292709661No Hit
CAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAAAAGGA6070.10662703724392823No Hit
ATATAGAGCAGCGGCGGTGTCGAGTAGATCTCGAATAGAAGGAAAGAAGT6020.10574872556976078No Hit
CTTTTGGAGTGTGGATTCGCACTCCTGCCGCTTACAGACCACAAAATGCC5940.10434342689109287No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5920.1039921022214259No Hit
TATCAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAAAA5900.1036407775517589No Hit
CTCAAGAACCGTTTGTCTTCCAAACATAAGACAGGAAATGTGAAACCACA5900.1036407775517589No Hit
GATAATATCCTCCTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT5870.10311379054725844No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTTAGATGTATAC5830.10241114120792447No Hit
CTGTCGTGCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGCTGT5740.10083018019442307No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5710.10030319318992262No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5710.10030319318992262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)