FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452638
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGATTTCCACAGAGTATGTAAATAATGCCTAGTTT14830.32763488703997456No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGTGTGTTCAGG13030.28786800931428647No Hit
CTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGG11760.2598102678078288No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA11120.2456709335053619No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCAAGG11070.24456629801298166No Hit
ATTGACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11010.2432407354221254No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC10840.23948497474803265No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATCAAACCCTAT9920.21915968168823652No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9910.21893875458976045No Hit
CTCTTATACAAGACCTTGGGCAATACCATGTGGGCGTTCACGGTGGTTTC9880.21827597329433235No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9810.21672948360500002No Hit
GGATAATAGGGTTTGATGCCTTTATCTAAAGGCAAATATTTGGTAAGGTT8800.19441584665891948No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTC8370.18491598142444957No Hit
ATATTAATGTTGACATAGCTGACCACTAATTCCCTGGATGCTGGGTCTTC8310.18359041883359328No Hit
GTTCTATGTTGGGGTGAGTTAATGAATCTGGCTACCTGGGTGGGAAGTAA8010.17696260587931195No Hit
CTCTTATATAGAATGCCAGATTTCCACAGAGTATGTAAATAATGCCTAGT7690.1698929387280785No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAACCTCCTTTTTTCA7690.1698929387280785No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTATTCG7570.16724181354636597No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATT7440.1643697612661774No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG7180.15862565670580023No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCTAG6970.15398618763780328No Hit
CCATAATAGTTGTCTGATTTTTAGGCCCATATTAATGTTGACATAGCTGA6930.15310247924389908No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGTGA6780.14978857276675842No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6670.14735837468352195No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6370.1407305617292406No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAACTTTGTAAGTTGACGA6210.13719572815362385No Hit
ATCAATAGGTGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6010.13277718618410297No Hit
GTTTTAAAGTAATGATTAACTGTGTGTTCAGGATAATAGGGTTTGATGCC6010.13277718618410297No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTTTGACACACC5960.13167255069172276No Hit
CAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA5600.12371917514658512No Hit
GATTAACTGTGTGTTCAGGATAATAGGGTTTGATGCCTTTATCTAAAGGC5580.12327732094963303No Hit
ATGATTAACTGTGTGTTCAGGATAATAGGGTTTGATGCCTTTATCTAAAG5520.12195175835877678No Hit
ATTTACATACTCTGTGGAAATCTGGCATTCTATATAAGAGAGAAACTACA5520.12195175835877678No Hit
CTATTATGGTTCCACATCTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5430.11996341447249237No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5390.1190797060785882No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA5330.11775414348773192No Hit
ATACAAGACCTTGGGCAATACCATGTGGGCGTTCACGGTGGTTTCCATGC5310.11731228929077982No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG5300.11709136219230377No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG5300.11709136219230377No Hit
CCTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCGAAAACAGTGTTTG5070.11201003892735475No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5050.11156818473040266No Hit
AATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCT5000.11046354923802243No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4930.10891705954869013No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4880.10781242405630989No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT4790.10582408017002551No Hit
CAATAGGCCTATTTACAGGAAGTTTTCGAAAACAGTGTTTGATTTTTTGT4670.10317295498831296No Hit
ATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGG4670.10317295498831296No Hit
AAGTTAAGGGAATAGCCCCATCTTTTGGTCTTATTAGGGTTCAAATGTAT4660.10295202788983691No Hit
GGGCAATACCATGTGGGCGTTCACGGTGGTTTCCATGCGACGTAGACGTG4630.10228924659440879No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATCAAAC4610.10184739239745667No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTA4590.10140553820050459No Hit
GAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGGTTAAAATT4550.10052182980660042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)