FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452638
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGTGTGTTCAGG12750.2816820505569572No Hit
CTTATATAGAATGCCAGATTTCCACAGAGTATGTAAATAATGCCTAGTTT11920.2633451013834455No Hit
CTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGG10460.23108974500594295No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCAAGG10370.22910140111965854No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC9470.2092179622568145No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA8670.1915437943787309No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8560.1891135962954944No Hit
GTTCTATGTTGGGGTGAGTTAATGAATCTGGCTACCTGGGTGGGAAGTAA8360.18469505432597352No Hit
GGATAATAGGGTTTGATGCCTTTATCTAAAGGCAAATATTTGGTAAGGTT8240.18204392914426099No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATCAAACCCTAT7780.17188128261436292No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7480.16525346966008156No Hit
ATATTAATGTTGACATAGCTGACCACTAATTCCCTGGATGCTGGGTCTTC7140.15774194831189606No Hit
CTCTTATATAGAATGCCAGATTTCCACAGAGTATGTAAATAATGCCTAGT7090.15663731281951582No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATT6930.15310247924389908No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6600.14581188499418962No Hit
ATTGACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6520.14404446820638128No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAACCTCCTTTTTTCA6430.14205612432009684No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTC6240.137858509449052No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTATTCG5910.13056791519934252No Hit
CTCTTATACAAGACCTTGGGCAATACCATGTGGGCGTTCACGGTGGTTTC5880.1299051339039144No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTTTGACACACC5730.1265912274267737No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG5520.12195175835877678No Hit
GTTTTAAAGTAATGATTAACTGTGTGTTCAGGATAATAGGGTTTGATGCC5360.11841692478316006No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGTGA5280.11664950799535169No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5200.11488209120754332No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAACTTTGTAAGTTGACGA5070.11201003892735475No Hit
CCTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCGAAAACAGTGTTTG5050.11156818473040266No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG5030.11112633053345057No Hit
GATTAACTGTGTGTTCAGGATAATAGGGTTTGATGCCTTTATCTAAAGGC5000.11046354923802243No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4990.11024262213954639No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA4820.10648686146545364No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG4750.10494037177612131No Hit
ATGATTAACTGTGTGTTCAGGATAATAGGGTTTGATGCCTTTATCTAAAG4710.10405666338221713No Hit
ATTTACATACTCTGTGGAAATCTGGCATTCTATATAAGAGAGAAACTACA4700.10383573628374108No Hit
CTATTATGGTTCCACATCTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4690.10361480918526504No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCTAG4670.10317295498831296No Hit
CCATAATAGTTGTCTGATTTTTAGGCCCATATTAATGTTGACATAGCTGA4620.10206831949593274No Hit
GTTCAGGATAATAGGGTTTGATGCCTTTATCTAAAGGCAAATATTTGGTA4540.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)