FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474260
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16560.3491755577109602No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTGAA15580.3285117867836208No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACT12910.2722135537468899No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10490.22118669084468437No Hit
TAATCTCTGGCTTGGAAGTAATGATTAACTGAATGTTCTGGATAATAAGG9620.2028423227765361No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8780.18513051912453085No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8740.1842870999030068No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA8680.18302197107072068No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8480.1788048749631004No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8200.172900940412432No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC8200.172900940412432No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8140.17163581158014593No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8070.1701598279424788No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT7830.16509931261333446No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7580.1598279424788091No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7540.15898452325728504No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC7470.15750853961961794No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT7410.15624341078733184No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7290.15371315312275965No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA6950.14654408973980515No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6930.14612238012904313No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6930.14612238012904313No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6670.14064015518913675No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6460.13621220427613545No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATTCAGTTAATCATTACT6440.1357904946653734No Hit
GCCACACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT6340.13368194661156327No Hit
TCCATAGAGTGTGTAAATAATCTCTGGCTTGGAAGTAATGATTAACTGAA6230.1313625437523721No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6210.1309408341416101No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6110.12883228608779995No Hit
TTCTTCTACTGTTCCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTC5980.12609117361784675No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5950.1254586092017037No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATATT5890.12419348036941762No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5800.12229578712098849No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC5620.11850040062413023No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5570.11744612659722516No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5510.11618099776493906No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA5200.1096444987981276No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5140.10837936996584152No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5020.10584911230126935No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5000.10542740269050732No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC4830.10184287099903007No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT4790.10099945177750602No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4760.10036688736136297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)