FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474260
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16440.34664530004638805No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTGAA12600.26567705478007847No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACT11320.23868763969130857No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9090.1916670180913423No Hit
TAATCTCTGGCTTGGAAGTAATGATTAACTGAATGTTCTGGATAATAAGG8420.1775397461308143No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8120.1712141019693839No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA7540.15898452325728504No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7400.15603255598195082No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC7280.15350229831737866No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6910.14570067051828112No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6750.14232699363218487No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT6610.13937502635685067No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6560.13832075232994562No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6490.1368447686922785No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6370.13431451102770633No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA6220.1311516889469911No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5660.11934381984565429No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5660.11934381984565429No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5550.11702441698646313No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5490.11575928815417703No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5450.11491586893265299No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5420.11428330451650993No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5420.11428330451650993No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC5370.11322903048960485No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5290.11154219204655674No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATTCAGTTAATCATTACT5280.11133133724117573No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5260.1109096276304137No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5240.11048791801965166No Hit
TTCTTCTACTGTTCCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTC5200.1096444987981276No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT5150.10859022477122254No Hit
TCCATAGAGTGTGTAAATAATCTCTGGCTTGGAAGTAATGATTAACTGAA5060.10669253152279341No Hit
GCCACACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT4870.10268629022055413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)