FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409719
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA27090.6611848608436514No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT23600.5760045299339304No Hit
GGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTA17490.4268779334129001No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC16420.4007624737930143No Hit
TCCCCAAGCGCCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG14240.3475552756889478No Hit
ATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAA14220.3470671362568004No Hit
GGATAACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAAT13810.3370602778977787No Hit
GTATACATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGTAAAA13450.3282737681191255No Hit
CCCTTAATTTTATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG11950.2916633107080706No Hit
CTTTTACACAATGTGGTTATCCTGCGCTAATGCCTTTGTATGCATGTATA11710.28580563752230187No Hit
AGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTT11710.28580563752230187No Hit
CCTTACCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAA11490.2804361037686805No Hit
CTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTAGTTT11450.2794598249043857No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC10150.24773076181480477No Hit
GTACAACACCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTTTTGTC10000.24406971607369932No Hit
ATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAAT9720.23723576402363572No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9670.2360154154432672No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACT9650.23552727601111983No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9530.23259843941823544No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC8940.21819832616988716No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8640.2108762346876762No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG8520.2079473980947918No Hit
GTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGC8310.20282193405724414No Hit
GTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC8270.2018456551929493No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8230.20086937632865454No Hit
CATTTGAACCCTCACAAAACCAAGCGTTGGGGCTACTCCCTTAATTTTAT8180.19964902774828602No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAAATTAAGGGAG8170.1994049580322123No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC7740.18890996024104326No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7690.18768961166067474No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7570.18476077506779034No Hit
TAACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCT7420.18109972932668486No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7410.18085565961061117No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7300.1781708927338005No Hit
GGCATAGACCGGGCCGTTGCCGGGCAACGGGGAAAAGGGTCAAATATTGT7290.17792682301772678No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCATTAAGGCA7090.1730454286962528No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7050.17206914983195798No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6950.169628452671221No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGAAA6920.16889624352299992No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTAGT6890.1681640343747788No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGATCATATTGTACTAAAA6880.16791996465870512No Hit
CCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCATTCTGTAAACACACAG6830.1666996160783366No Hit
GTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTGT6820.1664555463622629No Hit
TCCTAGTACAAAGACCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCATT6800.16596740693011552No Hit
ATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAATCGA6760.16499112806582072No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCATAGACCGGGCC6740.16450298863367332No Hit
CTATTGGTCGATTAACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGT6730.16425891891759964No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG6690.16328264005330484No Hit
GTACCTTACCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGA6660.16255043090508373No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6620.16157415204078893No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAACCGAGCAGCC6620.16157415204078893No Hit
CATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGTAAAAGGGGC6580.16059787317649413No Hit
CTTTTTACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6560.16010973374434673No Hit
CCTTTCTCTAAGGGCAAATATTTGGTAAGTTTAGGATAAAATCTAGCAGG6450.15742496686753604No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC6440.15718089715146233No Hit
CAATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATA6330.15449613027465164No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG6270.15303171197820947No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6260.15278764226213576No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAGTCTCCTCC6260.15278764226213576No Hit
GCCCAAAAGACCCACAATTCTTTGGCATACTTTCCAATCTATTGGTCGAT6210.15156729368176725No Hit
CTTTCCAATCTATTGGTCGATTAACAGGGAGTTTTCGAAAACATTGCTTG6200.15132322396569356No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGGCAGACGGAGAAGG6190.15107915424961987No Hit
GCCATGGAAACGAGGTGTATTTCCGAGAGAGGACAACAGCGTTGTCGGTT6160.15034694510139876No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6020.14692996907636696No Hit
GTACAATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5960.14546555077992476No Hit
CCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGA5930.14473334163170368No Hit
AATTAAGGGAGTAGCCCCAACGCTTGGTTTTGTGAGGGTTCAAATGTATA5890.1437570627674089No Hit
GACGTAGACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAACCGA5840.1425367141870404No Hit
CATACTGCGGAACTCCTTGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC5770.14082822617452448No Hit
GCCGTGCAACGGGGAAAAGGGTCAAATATTGTTTACGCAGAAAAGTCTTG5720.139607877594156No Hit
GCTTAGGGATCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACG5700.1391197381620086No Hit
GTGTATTTCCGAGAGAGGACAACAGCGTTGTCGGTTCCGATGAGTTTCGC5670.1383875290137875No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCCTCCGTGG5640.1376553198655664No Hit
ACATAGAATAGCTTGCCTTAATGCGGTATGGTGAGGTGAACAATGTTCCG5570.1359468318530505No Hit
ACTCATCGGAACCGACAACGCTGTTGTCCTCTCTCGGAAATACACCTCGT5450.1330179952601661No Hit
GTATAAAGAATTTGGAGCCTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG5390.1315535769637239No Hit
TTGGCATACTTTCCAATCTATTGGTCGATTAACAGGGAGTTTTCGAAAAC5380.13130950724765023No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGGCG5370.13106543753157654No Hit
CCTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACT5370.13106543753157654No Hit
ATGCCTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAG5340.13033322838335543No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGAGGTAAAAA5330.13008915866728174No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACC5300.12935694951906063No Hit
TCCAAAGGGTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTATA5270.1286247403708395No Hit
GGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTGTTACCAATT5250.12813660093869214No Hit
CTAATGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTGAT5180.12642811292617623No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTTTGGGTCACCATA5160.12593997349402883No Hit
TCCCTAAGCGCCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG5130.12520776434580771No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5120.12496369462973403No Hit
CCCCCAACTCCTCCCATTCTGTAAACACACAGTCTTTGAAGTACGCCTCA5120.12496369462973403No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA5110.12471962491366034No Hit
ACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTT5100.12447555519758664No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC5090.12423148548151294No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG5080.12398741576543923No Hit
ATACTGCGGAACTCCTTGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCG5050.12325520661721814No Hit
GTACAAAGACCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCATTCTGTA5040.12301113690114443No Hit
TTGCCCGGCAACGGCCCGGTCTATGCCAAGTGTTTGCTGACGCAACCCCC5020.12252299746899704No Hit
GTATACATTTGAACCCTCACAAAACCAAGCGTTGGGGCTACTCCCTTAAT5010.12227892775292334No Hit
ATCCAGTTGGCAGCACAACCGAGCAGCCATGGAAACGAGGTGTATTTCCG4930.12032637002433375No Hit
CGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGT4930.12032637002433375No Hit
CTGATGGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA4930.12032637002433375No Hit
CTTTCTCTAAGGGCAAATATTTGGTAAGTTTAGGATAAAATCTAGCAGGC4890.11935009116003895No Hit
CGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGGCA4850.11837381229574415No Hit
GTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAATCGACCAAT4820.11764160314752306No Hit
TACATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGTAAAAGGG4810.11739753343144937No Hit
ACATTGACCCGTATAAAGAATTTGGAGCCTCCGTGGAGTTACTCTCTTTT4810.11739753343144937No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4770.11642125456715458No Hit
GTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACTTTT4760.11617718485108088No Hit
CCAGTGGGGGTTGCGTCAGCAAACACTTGGCATAGACCGGGCCGTTGCCG4750.11593311513500716No Hit
GGTTCCACGCATGCGCTGATGGCCCATGGCCAAGCCCCAACCAGTGGGGG4740.11568904541893346No Hit
CTGTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATT4660.11373648769034386No Hit
GCTTGTATACATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGT4650.11349241797427016No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4640.11324834825819648No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCCATTCG4640.11324834825819648No Hit
GTGGTTATCCTGCGCTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG4600.11227206939390168No Hit
CCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCC4570.11153986024568056No Hit
GTCTTTGGGTATACATTTGAACCCTCACAAAACCAAGCGTTGGGGCTACT4560.11129579052960688No Hit
GATCCATACTGCGGAACTCCTTGCAGCTTGTTTTGCTCGCAGCCGGTCTG4530.11056358138138579No Hit
ACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGC4470.10909916308494358No Hit
GCATAGACCGGGCCGTTGCCGGGCAACGGGGAAAAGGGTCAAATATTGTT4460.10885509336886988No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4440.1083669539367225No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATTCCTATGGGAG4420.10787881450457508No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAGTCT4390.107146605356354No Hit
TAGATAAAGGCATTAAACCTTATTATCCTGAACATATAGTTAATCATTAC4370.10665846592420658No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4350.1061703264920592No Hit
CTTTTACACAATGTGGTTATCCTGCGCTAATGCCTTTGTATGCCTGTATA4340.10592625677598551No Hit
GAATTGTGGGTCTTTTGGGCTTTGCCGCCCCTTTTACACAATGTGGTTAT4330.10568218705991178No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACTTTTC4330.10568218705991178No Hit
GTTTTGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTAATGCC4190.10226521103488No Hit
ATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATAGCCGACTAC4160.10153300188665891No Hit
GCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4120.10055672302236411No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4120.10055672302236411No Hit
GGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC4110.10031265330629041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)