FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409719
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT24740.6038284775663321No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22750.5552586040676659No Hit
GGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTA18440.45006455643990145No Hit
ATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAA14770.36049097064085384No Hit
GTATACATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGTAAAA14740.35975876149263275No Hit
GGATAACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAAT13680.33388737158882065No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12510.3053312148081978No Hit
CTTTTACACAATGTGGTTATCCTGCGCTAATGCCTTTGTATGCATGTATA12430.30337865707960826No Hit
CCCTTAATTTTATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG12300.30020575077065015No Hit
CCTTACCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAA11950.2916633107080706No Hit
ATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAAT10980.2679885482489218No Hit
GTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC10850.26481564193996376No Hit
AGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTT10810.2638393630756689No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10680.26066645676671085No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACT10520.25676134130953165No Hit
CTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTAGTTT10370.25310029556842617No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC9960.2430934372094045No Hit
GTACAACACCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTTTTGTC9480.23137809083786692No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9450.23064588168964584No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC9370.22869332396105624No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9340.22796111481283515No Hit
CATTTGAACCCTCACAAAACCAAGCGTTGGGGCTACTCCCTTAATTTTAT8330.2033100734893915No Hit
GCTTAGGGATCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACG8220.20062530661258082No Hit
CTATTGGTCGATTAACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGT8040.19623205172325425No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGAAA7960.19427949399466465No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7940.19379135456251723No Hit
ATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAATCGA7870.19208286655000134No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7830.19110658768570654No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAAATTAAGGGAG7790.19013030882141174No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7770.18964216938926437No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7750.18915402995711694No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7590.18524891449993777No Hit
TAACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCT7220.1762183350052109No Hit
CCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCATTCTGTAAACACACAG7200.1757301955730635No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7160.1747539167087687No Hit
GTACCTTACCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGA7090.1730454286962528No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGATCATATTGTACTAAAA7060.17231321954803172No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTAGT7030.1715810103998106No Hit
GCCCAAAAGACCCACAATTCTTTGGCATACTTTCCAATCTATTGGTCGAT7000.1708488012515895No Hit
GTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTGT6990.1706047315355158No Hit
CCTTTCTCTAAGGGCAAATATTTGGTAAGTTTAGGATAAAATCTAGCAGG6870.1676758949426314No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCATAGACCGGGCC6780.16547926749796812No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6670.1627945006211574No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCATTAAGGCA6640.16206229147293633No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG6600.16108601260864153No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6500.15864531544790456No Hit
GATCCATACTGCGGAACTCCTTGCAGCTTGTTTTGCTCGCAGCCGGTCTG6480.15815717601575713No Hit
GTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGC6430.15693682743538864No Hit
GGCATAGACCGGGCCGTTGCCGGGCAACGGGGAAAAGGGTCAAATATTGT6400.15620461828716753No Hit
TCCTAGTACAAAGACCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCATT6350.15498426970679904No Hit
TTGCCCGGCAACGGCCCGGTCTATGCCAAGTGTTTGCTGACGCAACCCCC6310.15400799084250427No Hit
CTTTCCAATCTATTGGTCGATTAACAGGGAGTTTTCGAAAACATTGCTTG6310.15400799084250427No Hit
CATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGTAAAAGGGGC6220.15181136339784096No Hit
GTATAAAGAATTTGGAGCCTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG6150.15010287538532507No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6120.149370666237104No Hit
ATACTGCGGAACTCCTTGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCG6110.14912659652103027No Hit
CTTTTTACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6030.14717403879244068No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCCTCCGTGG6020.14692996907636696No Hit
AATTAAGGGAGTAGCCCCAACGCTTGGTTTTGTGAGGGTTCAAATGTATA6020.14692996907636696No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAGTCTCCTCC5980.1459536902120722No Hit
CCTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACT5900.1440011324834826No Hit
ATGCCTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAG5780.1410722958905982No Hit
GTACTAAAAATCAAGCAATGTTTTCGAAAACTCCCTGTTAATCGACCAAT5750.1403400867423771No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAACCGAGCAGCC5750.1403400867423771No Hit
GTACAATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5690.13887566844593488No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGGCG5680.1386315987298612No Hit
CAATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATA5670.1383875290137875No Hit
CTTTCTCTAAGGGCAAATATTTGGTAAGTTTAGGATAAAATCTAGCAGGC5520.13472648327268202No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGAGGTAAAAA5480.13375020440838722No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5480.13375020440838722No Hit
CTAATGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTGAT5460.1332620649762398No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5440.13277392554409242No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACC5410.1320417163958713No Hit
GTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACTTTT5400.13179764667979763No Hit
GCCGTGCAACGGGGAAAAGGGTCAAATATTGTTTACGCAGAAAAGTCTTG5310.1296010192351343No Hit
GTGGTTATCCTGCGCTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG5270.1286247403708395No Hit
CCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTCCC5270.1286247403708395No Hit
CCCCCAACTCCTCCCATTCTGTAAACACACAGTCTTTGAAGTACGCCTCA5270.1286247403708395No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5230.12764846150654474No Hit
ACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTT5190.12667218264224994No Hit
GCCATGGAAACGAGGTGTATTTCCGAGAGAGGACAACAGCGTTGTCGGTT5160.12593997349402883No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5160.12593997349402883No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG5100.12447555519758664No Hit
GTGTATTTCCGAGAGAGGACAACAGCGTTGTCGGTTCCGATGAGTTTCGC5090.12423148548151294No Hit
TCCAAAGGGTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTATA5080.12398741576543923No Hit
TTGGCATACTTTCCAATCTATTGGTCGATTAACAGGGAGTTTTCGAAAAC5070.12374334604936554No Hit
GGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC5040.12301113690114443No Hit
TTCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5030.12276706718507074No Hit
CCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGA5030.12276706718507074No Hit
GGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACCTCTGTTACCAATT5020.12252299746899704No Hit
ACATTGACCCGTATAAAGAATTTGGAGCCTCCGTGGAGTTACTCTCTTTT5020.12252299746899704No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5010.12227892775292334No Hit
GAAATACACCTCGTTTCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCC5000.12203485803684966No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5000.12203485803684966No Hit
GACGTAGACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAACCGA4960.12105857917255486No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGACCCGTAT4910.11983823059218636No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACTTTTC4890.11935009116003895No Hit
ACATAGAATAGCTTGCCTTAATGCGGTATGGTGAGGTGAACAATGTTCCG4850.11837381229574415No Hit
CATACTGCGGAACTCCTTGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4820.11764160314752306No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4770.11642125456715458No Hit
CTTTTGGGCTTTGCCGCCCCTTTTACACAATGTGGTTATCCTGCGCTAAT4760.11617718485108088No Hit
GGTTCCACGCATGCGCTGATGGCCCATGGCCAAGCCCCAACCAGTGGGGG4730.11544497570285976No Hit
GTACAAAGACCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCATTCTGTA4720.11520090598678608No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4710.11495683627071236No Hit
GTCTTTGGGTATACATTTGAACCCTCACAAAACCAAGCGTTGGGGCTACT4660.11373648769034386No Hit
CTTTTACACAATGTGGTTATCCTGCGCTAATGCCTTTGTATGCCTGTATA4640.11324834825819648No Hit
GCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4580.11178392996175428No Hit
TACATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGTAAAAGGG4560.11129579052960688No Hit
CTGATGGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA4550.11105172081353318No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATTCCTATGGGAG4550.11105172081353318No Hit
GTATACATTTGAACCCTCACAAAACCAAGCGTTGGGGCTACTCCCTTAAT4520.11031951166531206No Hit
ATGTGGTTATCCTGCGCTAATGCCTTTGTATGCATGTATACAAGCTAAGC4520.11031951166531206No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCCATTCG4510.11007544194923838No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG4500.10983137223316468No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC4450.1086110236527962No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4440.1083669539367225No Hit
TAGATAAAGGCATTAAACCTTATTATCCTGAACATATAGTTAATCATTAC4420.10787881450457508No Hit
GAATTGTGGGTCTTTTGGGCTTTGCCGCCCCTTTTACACAATGTGGTTAT4400.1073906750724277No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTTTGGGTCACCATA4380.10690253564028028No Hit
CCCCACTGGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCT4370.10665846592420658No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4370.10665846592420658No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4310.1051940476277644No Hit
GACCAATAGATTGGAAAGTATGCCAAAGAATTGTGGGTCTTTTGGGCTTT4260.1039736990473959No Hit
AGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACTTTTCTGCGTA4260.1039736990473959No Hit
ACTCATCGGAACCGACAACGCTGTTGTCCTCTCTCGGAAATACACCTCGT4260.1039736990473959No Hit
GCTTGTATACATGCATACAAAGGCATTAGCGCAGGATAACCACATTGTGT4230.1032414898991748No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGGCAGACGGAGAAGG4190.10226521103488No Hit
TGGTTATCCTGCGCTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGG4110.10031265330629041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)