FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190687
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC4200.22025623141588047No Hit
GTATGCAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCGTGTTCAGG3970.20819458064786797No Hit
CTTATATAGAATGCCAGATTTCCACAGAGTATGCAAATAATGCCTAGTTT3880.20347480426038483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3820.2003282866687294No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA3770.1977061886756832No Hit
GGGCAATACCATGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG3720.195084090682637No Hit
GCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGCCTTACTTTT3530.18512011830906144No Hit
ATTTAGAAGACCCAGCATCCAGGGAATTAGTGGTCAATTATGTCAACATT3240.16991194994939351No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTATTCG2960.1552282011883348No Hit
TATCAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA2950.1547037815897256No Hit
ATCAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG2880.15103284439946088No Hit
ATGGAAACGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTTCCG2790.14631306801197774No Hit
AAACTTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAA2640.13844677403283917No Hit
GAAACGTTTTGTAAGTTAACGAAAAAGTGAAAGCTCGTTTAGCTTGAATA2530.13267815844813752No Hit
CTCACAAGACCAAGAGATGGGGCTATTCCCTTAACTTCATGGGCTATGTA2450.12848280165926362No Hit
ATCCTAACCTTACCAAATATTTGCCGTTAGATAAAGGCATTAAACCCTAT2440.12795838206065438No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT2370.12428744487038969No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT2230.11694557048986035No Hit
CCTATTCTCATCCCCCAACTCCTCCCAGTCCTTAAACACACAGTCTTTGA2160.11327463329959568No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGCAGATGAGAAGGCACAG2120.11117695490515872No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC2110.11065253530654948No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA2080.10907927651072176No Hit
ACCACGGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAG2070.10855485691211252No Hit
GTTTCAGGCCCATATTAATGTTGACATAATTGACCACTAATTCCCTGGAT2070.10855485691211252No Hit
ATATTAATGTTGACATAATTGACCACTAATTCCCTGGATGCTGGGTCTTC2020.10593275891906631No Hit
CCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACCG2010.10540833932045708No Hit
TAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAG2010.10540833932045708No Hit
GTCCTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA1990.1043595001232386No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT1940.10173740213019242No Hit
CTCTTATATAGAATGCCAGATTTCCACAGAGTATGCAAATAATGCCTAGT1910.1001641433343647No Hit
GGATAATAGGGTTTAATGCCTTTATCTAACGGCAAATATTTGGTAAGGTT1910.1001641433343647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)