FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575684
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44630.775251700585738No Hit
GTGCTGGTGGACCAATTGATCCTAAAACTGGTAGGAAGATGTATGAGCCT40270.6995157065334454No Hit
GTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG34740.6034560627010651No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAGTGTCTAGTTT25960.45094183614621913No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT22160.38493340096302836No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA21730.37746402540282514No Hit
ATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT21040.36547828322482473No Hit
GTCTTTAACGTCTCTAACCTAGCTTGATTAGCTAGGTCTTTAAGTCTATT20770.36078821019865065No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20770.36078821019865065No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAGTGTCTAGT19900.3456757526698675No Hit
ATACTATACTTAAGACTAGAGGTTATAACAAGTTAGGTGATGCCGTTGAT15290.2655970984081545No Hit
TGGTAATACTATACTTAAGACTAGAGGTTATAACAAGTTAGGTGATGCCG14540.25256911777989316No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14210.24683680630345814No Hit
TGATGATGGTGATCGGGTAATCGACTTAGTCTTTAACGTCTCTAACCTAG13440.23346141285844318No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT12600.21887007455479046No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT12550.21800154251290638No Hit
GTATGGTAATACTATACTTAAGACTAGAGGTTATAACAAGTTAGGTGATG12440.21609077202076138No Hit
GAGTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11940.20740545160192048No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCATG11870.2061895067432828No Hit
GTCTTTAAGTCTATTTGAATGGTTAGCATAGATGGTTTCCATACGTGTAC11790.20479985547626825No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11600.2014994337171087No Hit
ATTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC11410.19819901195794914No Hit
GGTTAGCATAGATGGTTTCCATACGTGTACCAGAGGATAGAGCGTGCGCA11370.1975041863244419No Hit
TCTCTAACCTAGCTTGATTAGCTAGGTCTTTAAGTCTATTTGAATGGTTA11350.19715677350768826No Hit
ATGATGGTGATCGGGTAATCGACTTAGTCTTTAACGTCTCTAACCTAGCT11100.1928141132982678No Hit
TCCTAAAACTGGTAGGAAGATGTATGAGCCTACTAATAGGACTAGGGTTG10620.18447620569618056No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGTTGAGAGTCCAA10300.17891760062812237No Hit
GGTAATCGACTTAGTCTTTAACGTCTCTAACCTAGCTTGATTAGCTAGGT10270.17839648140299194No Hit
GCATAGATGGTTTCCATACGTGTACCAGAGGATAGAGCGTGCGCATCAAC10100.175443472460586No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAACGTAAGA9850.17110081225116558No Hit
TGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATA9300.1615469597904406No Hit
ACTTAAGACTAGAGGTTATAACAAGTTAGGTGATGCCGTTGATGCGCACG9290.16137325338206376No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9280.16119954697368696No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA9280.16119954697368696No Hit
TTATATGCCTGTATACAATCTAAGAAAGCTTTCACTTTCTCGCCAACTTA9270.16102584056531014No Hit
GTATAGTATTACCATACTTATCAACCCTAGTCCTATTAGTAGGCTCATAC9270.16102584056531014No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9160.15911507007316514No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACACCCTCCTTTC8960.15564094190562877No Hit
AACCTAGCTTGATTAGCTAGGTCTTTAAGTCTATTTGAATGGTTAGCATA8930.15511982268049834No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACACCCTCCTTTCCTCAC8840.15355646500510697No Hit
CCTGTATACAATCTAAGAAAGCTTTCACTTTCTCGCCAACTTACAAGGCC8730.15164569451296198No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8600.14938751120406335No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGAGGTCTCCATGC8440.14660820867003424No Hit
GTATACAGGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8410.1460870894449038No Hit
GACTAGGGTTGATAAGTATGGTAATACTATACTTAAGACTAGAGGTTATA8350.14504485099464287No Hit
TCCACAGAGTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCA8300.14417631895275881No Hit
ATTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACCACA8270.14365519972762836No Hit
GTTATAACCTCTAGTCTTAAGTATAGTATTACCATACTTATCAACCCTAG8220.14278666768574425No Hit
CATTTAAACCCTAATAAAACCAAGCGATGGGGCTACTCCCTTAACTTCAT8190.14226554846061382No Hit
CTCTATCCTCTGGTACACGTATGGAAACCATCTATGCTAACCATTCAAAT8020.1393125395182079No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACACTATT7980.13861771388470062No Hit
CTCTAACCTAGCTTGATTAGCTAGGTCTTTAAGTCTATTTGAATGGTTAG7970.13844400747632382No Hit
GTATGAGCCTACTAATAGGACTAGGGTTGATAAGTATGGTAATACTATAC7740.134448760083657No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAACGATGTCAACGACCGACCT7680.13340652163339609No Hit
GTATTACCATACTTATCAACCCTAGTCCTATTAGTAGGCTCATACATCTT7660.13305910881664246No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7500.13027980628261338No Hit
GGTTATAACAAGTTAGGTGATGCCGTTGATGCGCACGCTCTATCCTCTGG7320.12715309093183066No Hit
ATCCTCTGGTACACGTATGGAAACCATCTATGCTAACCATTCAAATAGAC7320.12715309093183066No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCCTGTATA7320.12715309093183066No Hit
GGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7220.12541602684806247No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT7190.12489490762293202No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7170.1245474948061784No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7060.1226367243140334No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGCAGACCCACAATTCGTTGACATACT7040.12228931149727976No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT6720.11673070642922159No Hit
ACTTAGTCTTTAACGTCTCTAACCTAGCTTGATTAGCTAGGTCTTTAAGT6660.11568846797896067No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6600.11464622952869978No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6570.11412511030356932No Hit
GTCTATTGACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA6540.11360399107843885No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAT6460.11221433981142433No Hit
ATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATATT6380.11082468854440979No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6290.10926133086901842No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6260.10874021164388797No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACACTATTTACAT6230.10821909241875752No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6180.10735056037687342No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT6160.10700314756011979No Hit
ATACTTAAGACTAGAGGTTATAACAAGTTAGGTGATGCCGTTGATGCGCA6130.10648202833498935No Hit
GGGTTGATAAGTATGGTAATACTATACTTAAGACTAGAGGTTATAACAAG6120.10630832192661252No Hit
TTCCATACGTGTACCAGAGGATAGAGCGTGCGCATCAACGGCATCACCTA6100.10596090910985888No Hit
CTTTAATCCTGAATGGCAAACACCCTCCTTTCCTCACATTCATTTACAGG6100.10596090910985888No Hit
CTATTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACCA6080.10561349629310524No Hit
GCTAGATTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCAT6070.10543978988472842No Hit
CTATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATA6010.10439755143446752No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5900.10248678094232254No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5790.10057601045017754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)