FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375344
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45731.2183490344856984No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGA9600.25576537789334586No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGAGAAAGGCATTAAACCTTAT7860.2094079031501769No Hit
GGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATATTTGGTAAGGTT7600.20248092416556546No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7380.19661963425550963No Hit
GGTTAGGATAGAATCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG7380.19661963425550963No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7080.18862696619634256No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6930.184630632166759No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC6840.18223283174900892No Hit
GGACCATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTGCCTGTCA5800.1545249158105631No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5730.1526599599300908No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5620.14972931497506287No Hit
GTATAGGCAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCACACAG5620.14972931497506287No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5560.14813078136322946No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCTCTTTTCCTCAC5470.14573298094547935No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5070.13507609019992328No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATATTTGGTA4870.12974764482714524No Hit
AGTCTGTACAACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT4850.12921480028986743No Hit
GGATAGAATCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCGTTAAC4840.12894837802122852No Hit
ATATGGTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4830.12868195575258962No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4730.12601773306620062No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4690.12495204399164499No Hit
CTCTTATATAAAATACCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT4650.12388635491708938No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4630.1233535103798116No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4620.12308708811117268No Hit
CTTATATAAAATACCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT4600.12255424357389488No Hit
CTTTAATCCTGAATGGAAAACTCCCTCTTTTCCTCACATACATTTGAAAG4440.11829148727567244No Hit
GTACAACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC4380.11669295366383903No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAAAGCTTGGTGGAATGTGCTTG4250.1132294641715333No Hit
GTGTCAACAATACCTTAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4230.11269661963425551No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATAT4220.11243019736561662No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4170.1110980860224221No Hit
ACTATATGTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATA4160.1108316637537832No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCTCTTTTC4160.1108316637537832No Hit
CCCTTTACCCAATGTGGTTACCCAGCATTGATGCCTTTGTATGCATGTAT4140.11029881921650539No Hit
CCTCCCGGTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4140.11029881921650539No Hit
ATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCG4120.1097659746792276No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4050.10790101879875527No Hit
GTTTAATGCCTTTCTCTAAAGGCAAATATTTGGTAAGGTTAGGATAGAAT3980.10603606291828295No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCT3960.10550321838100517No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTCCG3950.10523679611236626No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3920.10443752930644956No Hit
ATCCCAATGTTAGTATTCCTTGGACTCACAAGGTGGGAAATTTTACTGGG3910.10417110703781064No Hit
TGCCTATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA3880.10337184023189393No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3830.10203972888869943No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA3790.10097403981414382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)