FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375344
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45331.2076921437401422No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGA9370.2496376657146511No Hit
GGTTAGGATAGAATCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG7570.20168165735964874No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7420.1976853233300652No Hit
GGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATATTTGGTAAGGTT7260.1934225670318428No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGAGAAAGGCATTAAACCTTAT7090.18889338846498147No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6780.1806342981371755No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6430.17130951873481393No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6380.16997740739161943No Hit
GTATAGGCAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCACACAG5670.1510614263182574No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5660.1507950040496185No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATATTTGGTA5230.1393388464981457No Hit
GGACCATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTGCCTGTCA5220.1390724242295068No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC5200.138539579692229No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5160.13747389061767337No Hit
GTGTCAACAATACCTTAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5120.13640820154311778No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCTCTTTTCCTCAC5120.13640820154311778No Hit
ATATGGTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5060.13480966793128435No Hit
CTCTTATATAAAATACCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT4920.13107975617033973No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4870.12974764482714524No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4820.1284155334839507No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATAT4660.12415277718572829No Hit
AGTCTGTACAACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT4650.12388635491708938No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4620.12308708811117268No Hit
GGATAGAATCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCGTTAAC4610.12282066584253379No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4600.12255424357389488No Hit
CTTTAATCCTGAATGGAAAACTCCCTCTTTTCCTCACATACATTTGAAAG4470.11909075408158916No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCTCTTTTC4420.11775864273839463No Hit
CTTATATAAAATACCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT4410.11749222046975574No Hit
GTACAACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC4390.11695937593247795No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4340.11562726458928343No Hit
CCCTTTACCCAATGTGGTTACCCAGCATTGATGCCTTTGTATGCATGTAT4200.1118973528283388No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4120.1097659746792276No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCT4120.1097659746792276No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4070.10843386333603308No Hit
ATTCTATCCTAACCTTACCAAATATTTGCCTTTAGAGAAAGGCATTAAAC4050.10790101879875527No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAAAGCTTGGTGGAATGTGCTTG4040.10763459653011637No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG4020.10710175199283858No Hit
GTTTAATGCCTTTCTCTAAAGGCAAATATTTGGTAAGGTTAGGATAGAAT4000.10656890745556076No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3880.10337184023189393No Hit
ACTATATGTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAAGGCAAATA3870.10310541796325504No Hit
ATCCCAATGTTAGTATTCCTTGGACTCACAAGGTGGGAAATTTTACTGGG3860.10283899569461614No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3820.10177330662006054No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3790.10097403981414382No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3770.10044119527686601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)