FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420595
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17100.4065668873857274No Hit
CTTTTATATAAGACACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT13320.3166942070162508No Hit
ATATAAGACACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA11780.2800794113101677No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10380.2467932333955468No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG9790.2327654869886708No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9750.23181445333396736No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8370.19900379224669812No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6560.15596951937136674No Hit
GCTACGTGTTGTCTCTCTTTTATATAAGACACCCGCCTTCCAAAGAGTAT6360.15121435109784948No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT6100.145032632342277No Hit
GTTGATATTCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC6050.14384384027389768No Hit
GTTAATATTCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC5850.13908867200038041No Hit
GTTAGTATTCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC5560.13219367800378035No Hit
GTGTTGTCTCTCTTTTATATAAGACACCCGCCTTCCAAAGAGTATGTAAA5390.12815178497129065No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5150.12244558304306995No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5130.12197006621571821No Hit
TTACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAG5110.12149454938836649No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA4990.11864144842425611No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT4910.1167393811148492No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT4910.1167393811148492No Hit
GCTGACACCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAA4740.1126974880823595No Hit
GGTCAAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT4690.1115086960139802No Hit
CTCTTTTATATAAGACACCCGCCTTCCAAAGAGTATGTAAATAATGTCTC4650.11055766235927673No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4620.10984438711824915No Hit
TTGCTCACACCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAG4480.10651576932678705No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA4390.10437594360370428No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT4350.10342490994900082No Hit
GTTAGTATCCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC4340.10318715153532496No Hit
ACTAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA4290.10199835946694563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)