FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420595
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16870.4010984438711825No Hit
CTTTTATATAAGACACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT13430.3193095495666853No Hit
ATATAAGACACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA11530.27413545096827113No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG10630.25273719373744336No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10170.24180030670835365No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9370.22277963361428452No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7600.1806963943936566No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT6480.15406745206195982No Hit
GTGTTGTCTCTCTTTTATATAAGACACCCGCCTTCCAAAGAGTATGTAAA6250.14859900854741495No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6200.14741021647903563No Hit
GTTAGTATTCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC5950.14146625613713903No Hit
GTTAATATTCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC5830.13861315517302866No Hit
GCTACGTGTTGTCTCTCTTTTATATAAGACACCCGCCTTCCAAAGAGTAT5700.13552229579524244No Hit
GTTGATATTCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC5360.12743850973026308No Hit
TTACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAG5280.12553644242085615No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT4960.11792817318322853No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT4890.11626386428749748No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4860.11555058904646988No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4820.11459955539176643No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4710.11198421284133192No Hit
CTCTTTTATATAAGACACCCGCCTTCCAAAGAGTATGTAAATAATGTCTC4650.11055766235927673No Hit
GCTGACACCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAA4470.10627801091311119No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA4460.10604025249943533No Hit
GGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA4350.10342490994900082No Hit
GTTAGTATCCCTTGGACCCATAAGGTGGGAAACTTTACGGGGCTTTATTC4320.10271163470797323No Hit
GGTCAAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT4300.1022361178806215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)