FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473410
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAGGATCTAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC20890.4412665554170804No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA19960.42162184998204516No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT16850.35592826514015335No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15470.3267780570752625No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14780.31220295304281703No Hit
GTCTTTAAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14700.3105130859086204No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGAG14330.30269745041296126No Hit
ATTCTATGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGTAA14300.3020637502376376No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13910.29382564795842925No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT13190.2786168437506601No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12940.2733360089562958No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12830.2710124416467755No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA12210.2579159713567521No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11590.24481950106672862No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG11590.24481950106672862No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT11450.24186223358188463No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11230.23721509896284404No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT10980.23193426416847976No Hit
ATATCCAGGAGGAGTACGAATCCACACTCCAAAAGACACCAAATATTCAA10010.2114446251663463No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT9850.20806489089795316No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9600.20278405610358888No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT9500.20067172218584312No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG9480.20024925540229396No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9290.19623582095857714No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8940.18884265224646712No Hit
CGTTAATATGGGCCTAAAAGTCAGACAACTATTGTGGTTTCACATTTCCT8710.18398428423565197No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAATCCACACGTAGCGCC8380.1770135823070911No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8320.17574618195644368No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG8250.17426754821402168No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8220.17363384803869797No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8080.17067658055385396No Hit
GTCTATATCCAGGAGGAGTACGAATCCACACTCCAAAAGACACCAAATAT8020.16940918020320653No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7900.16687437950191167No Hit
ATCCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATTAAT7730.16328341184174394No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7710.16286094505819482No Hit
GCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG7650.16159354470754736No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7590.16032614435689993No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7460.1575801102638305No Hit
CTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCT7450.15736887687205595No Hit
ATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG7400.15631270991318308No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT7380.15589024312963393No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7290.15398914260366278No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT7290.15398914260366278No Hit
ATATAAAAGAGAATCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7210.15229927546946623No Hit
ATGTAAGACCTTGGGCAGGTTCCCGGGGGCGTTCACGGTGGTCTCCATGC7190.15187680868591708No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7140.1508206417270442No Hit
CTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGAC7080.14955324137639678No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7020.14828584102574935No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT6890.14553980693267993No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT6770.14300500623138507No Hit
GGGTAAAGGTGCAGATACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA6750.14258253944783592No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA6730.14216007266428676No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTAGATCCTGGATCGTC6660.14068143892186477No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC6630.14004773874654106No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT6630.14004773874654106No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG6620.13983650535476647No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6600.13941403857121734No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6600.13941403857121734No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6590.13920280517944278No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC6380.13476690395217675No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC6360.13434443716862762No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6330.1337107369933039No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6280.13265457003443104No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6170.13033100272491074No Hit
ACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATT6090.12864113559071416No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6050.1277962020236159No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6030.12737373524006676No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT6020.12716250184829217No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5960.12589510149764474No Hit
ATTCTGAGCAGGGCTCACAGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5940.1254726347140956No Hit
AGTCTGTACAACATCTTGATTCCATTTATGCCGCTGTTACCAATTTTCTT5910.1248389345387719No Hit
GTAAACACACAGTCTTTAAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5890.12441646775522275No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5890.12441646775522275No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5880.12420523436344817No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5860.12378276757989903No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5800.1225153672292516No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5730.1210367334868296No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5670.11976933313618218No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTT5580.11786823261021102No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5560.11744576582666189No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5550.11723453243488731No Hit
GTACGAATCCACACTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCT5540.11702329904311273No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5490.11596713208423988No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5480.1157558986924653No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5450.11512219851714159No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5390.11385479816649416No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5340.1127986312076213No Hit
GTACAACATCTTGATTCCATTTATGCCGCTGTTACCAATTTTCTTTTGTC5330.11258739781584673No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5310.11216493103229758No Hit
ACAGTATTCTGAGCAGGGCTCACAGTTCCTGAACTGGAGCCACCAGCAGG5280.11153123085697388No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5270.1113199974651993No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5230.11047506389810101No Hit
CTTTTGGAGTGTGGATTCGTACTCCTCCTGGATATAGACCACAAAATGCC5230.11047506389810101No Hit
GTGCAGATACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5220.11026383050632643No Hit
ATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTAT5200.10984136372277731No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5190.10963013033100273No Hit
GAATTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGACGATCCAGG5160.108996430155679No Hit
CTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCT5140.10857396337212986No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5130.10836272998035529No Hit
CTGTAGATCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTAC5130.10836272998035529No Hit
CTCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5100.10772902980503159No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA5080.10730656302148243No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC4970.10498299571196215No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC4890.10329312857776558No Hit
GGCCGAGACAGTATTCTGAGCAGGGCTCACAGTTCCTGAACTGGAGCCAC4880.10308189518599101No Hit
CAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTT4870.10287066179421643No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4840.10223696161889272No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4840.10223696161889272No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA4810.10160326144356902No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4810.10160326144356902No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4800.10139202805179444No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCCGAGACAGTA4760.10054709448469613No Hit
GTTGATAAGATAGGGGCATTTTGTGGTCTATATCCAGGAGGAGTACGAAT4740.10012462770114698No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4740.10012462770114698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)