FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473410
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAGGATCTAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC21280.4495046576962886No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA18730.39564014279377285No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16640.35149236391288735No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT15220.32149722228089817No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGAG14610.3086119853826493No Hit
ATTCTATGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGTAA14530.3069221182484527No Hit
GTCTTTAAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14510.30649965146490354No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13970.29509304830907673No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13880.29319194778310553No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13810.29171331404068357No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT13000.27460340930694327No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11740.2479880019433472No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11670.24650936820092517No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT11610.24524196785027777No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG11380.24038359983946261No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT11200.23658139878752033No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10940.23108933060138145No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10670.22538602902346805No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG9840.20785365750617857No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9820.20743119072262942No Hit
ATATCCAGGAGGAGTACGAATCCACACTCCAAAAGACACCAAATATTCAA9420.19898185505164656No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT9390.19834815487632285No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9240.19517965399970427No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT9190.19412348704083143No Hit
GTCTATATCCAGGAGGAGTACGAATCCACACTCCAAAAGACACCAAATAT8730.1844067510192011No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8490.1793371496166114No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8260.17447878160579625No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG8230.17384508143047253No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG8200.17321138125514882No Hit
CGTTAATATGGGCCTAAAAGTCAGACAACTATTGTGGTTTCACATTTCCT8090.17088781394562852No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAATCCACACGTAGCGCC8050.17004288037853024No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT7800.16476204558416593No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7630.16117107792399823No Hit
ATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG7540.1592699773980271No Hit
ATCCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATTAAT7420.1567351766967322No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT7250.1531442090365645No Hit
CTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCT7170.15145434190236792No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7150.1510318751188188No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7110.1501869415517205No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6880.14532857354090534No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6870.14511734014913077No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6830.1442724065820325No Hit
ATATAAAAGAGAATCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6810.14384993979848334No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6770.14300500623138507No Hit
GCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG6760.1427937728396105No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTAGATCCTGGATCGTC6740.14237130605606135No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT6700.14152637248896305No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6670.14089267231363933No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6650.1404702055300902No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG6510.1375129380452462No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC6490.13709047126169704No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6390.1349781373439513No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6360.13434443716862762No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6340.13392197038507847No Hit
CTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGAC6330.1337107369933039No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6250.13202086985910733No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6240.13180963646733274No Hit
GTACGAATCCACACTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCT6190.1307534695084599No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC6170.13033100272491074No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6160.13011976933313618No Hit
GGGTAAAGGTGCAGATACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA6160.13011976933313618No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA6140.12969730254958706No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG6100.12885236898248875No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTT5930.12526140132232103No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5920.12505016793054646No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5810.12272660062102618No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5800.1225153672292516No Hit
GTACAACATCTTGATTCCATTTATGCCGCTGTTACCAATTTTCTTTTGTC5770.12188166705392788No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5770.12188166705392788No Hit
AGTCTGTACAACATCTTGATTCCATTTATGCCGCTGTTACCAATTTTCTT5760.1216704336621533No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5720.12082550009505502No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5640.11913563296085845No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5620.1187131661773093No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5570.11765699921843645No Hit
GTACACCATCTTGAGTCCATTTATGCCGCTGTTACCAATTTTCTTATGTC5560.11744576582666189No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5530.11681206565133816No Hit
ATGTAAGACCTTGGGCAGGTTCCCGGGGGCGTTCACGGTGGTCTCCATGC5530.11681206565133816No Hit
ACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATT5490.11596713208423988No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5460.11533343190891615No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5430.11469973173359245No Hit
ATTCTGAGCAGGGCTCACAGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5260.11110876407342474No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5240.11068629728987558No Hit
CTTTTGGAGTGTGGATTCGTACTCCTCCTGGATATAGACCACAAAATGCC5240.11068629728987558No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT5220.11026383050632643No Hit
GTGCAGATACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5220.11026383050632643No Hit
CTCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5220.11026383050632643No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5200.10984136372277731No Hit
GTAAACACACAGTCTTTAAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5180.10941889693922816No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5180.10941889693922816No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5140.10857396337212986No Hit
ATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTAT5120.10815149658858073No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5080.10730656302148243No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5070.10709532962970786No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5050.1066728628461587No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5030.10625039606260958No Hit
CTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCT5030.10625039606260958No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA5020.106039162670835No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5020.106039162670835No Hit
GAATTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGACGATCCAGG4990.10540546249551129No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTA4970.10498299571196215No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4950.10456052892841301No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT4920.10392682875308928No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4890.10329312857776558No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4890.10329312857776558No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCCGAGACAGTA4870.10287066179421643No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG4850.10244819501066729No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC4840.10223696161889272No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4750.10033586109292156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)