FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427010
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19680.4608791363199925No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12890.30186646682747476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11590.2714222149364183No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11570.27095384183040205No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11310.26486499145219083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGCG11100.25994707383902016No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG10570.24753518652958945No Hit
CTTATATAAAATACCAGCCTTCCACAGAATATGCAAATAATGTCTAGTTT9410.22036954638064685No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9210.2156858153204843No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCA9120.21357813634341116No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCA8780.20561579354113485No Hit
CTCCAAAAGACACCAAATACTCTAGAACAACTTGTCTTCCAAAAGTAAGA8110.18992529448959042No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA8110.18992529448959042No Hit
GTAGTAGGCTATGTCAATGTTAACATGGGCCTAAAATTCAGACAACTATT7780.18219713824032224No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7610.1782159668391841No Hit
CTCTAGAACAACTTGTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA7590.17774759373316784No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7380.17282967611999717No Hit
GGAACATATTGTGCAAAAATTGAAACAATGTTTTCGGAAACTTCCTGTAA7150.16744338540081027No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7080.1658040795297534No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGAAGATTAAAATTGAT7040.16486733331772088No Hit
CTGTAAGAGGGCCCACATATTGTTCACATCTGTTGACAATGTCTTCTTTC6940.16252546778763963No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6830.15994941570455024No Hit
GAATATGCAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA6610.15479731153837148No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAATATGCAAATAATGTCTAGT6600.15456312498536334No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6570.15386056532633896No Hit
GCCTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC6380.14941102081918456No Hit
CTTTTACACAATGTGGTTACCCAGCATTGATGCCTTTATATGCATGTATA6320.1480059015011358No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGATTGCCGTG6310.14777171494812769No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTACTGCCTCCACAAATCGGCAGT6250.14636659563007892No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6200.1451956628650383No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6160.14425891665300578No Hit
GTTGACAATGTCTTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6140.14379054354698953No Hit
ATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTCACGGTGGTTTCCATGC6090.1426196107819489No Hit
GTATACATGCATATAAAGGCATCAATGCTGGGTAACCACATTGTGTAAAA5970.13980937214585137No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5960.13957518559284324No Hit
TCCTCAGGCCGTGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTGG5920.13863843938081075No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGCACAATATGTT5830.13653076040373763No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCATTAAC5820.1362965738507295No Hit
CCATACAGCAATAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5800.13582820074471325No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5750.13465726797967262No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCAGGATTCAGCGCCGACGGG5660.13254958900259947No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5450.12763167138942882No Hit
TGTTAACATTGACATAGCCTACTACTAATTCCCTGGATGCTGGATCTTCC5390.12622655207138006No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT5370.12575817896536381No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5350.12528980585934754No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5310.12435305964731505No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5300.12411887309430691No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5160.12084026135219315No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5150.12060607479918502No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT5070.11873258237512002No Hit
CCAAAAGACACCAAATACTCTAGAACAACTTGTCTTCCAAAAGTAAGACA5000.11709327650406313No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG4980.11662490339804689No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4930.11545397063300626No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4920.11521978407999812No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4880.11428303786796562No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG4800.11240954544390061No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAATATGCA4740.11100442612585186No Hit
CATATAAAGGCATCAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCA4640.10866256059577058No Hit
GCAATAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4630.10842837404276247No Hit
ATGTTAACATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCT4610.10796000093674621No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4600.10772581438373809No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCAATAAGGCA4580.10725744127772184No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGCACAAT4560.10678906817170558No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCAAGC4560.10678906817170558No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG4540.10632069506568932No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAA4540.10632069506568932No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4490.10514976230064868No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4480.10491557574764057No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTC4420.10351045642959182No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG4390.10280789677056743No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4350.10187115055853492No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4350.10187115055853492No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTG4340.1016369640055268No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAATATGCAAA4330.10140277745251869No Hit
GGATAGGGGCATTTGGTGGTCTGTAAGCTTGAGGAGTGCGAATCCACACT4300.10070021779349429No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4290.10046603124048617No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAATCCCTTTTTACC4290.10046603124048617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)