FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427010
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16330.3824266410622702No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGCG12680.29694854921430414No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11510.26954872251235334No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10480.24542750755251636No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10360.24261726891641883No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10090.23629423198519942No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCA9140.2140465094494274No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG9110.21334394979040305No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8990.21053371115430553No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCA8580.20093206248097234No Hit
CTCCAAAAGACACCAAATACTCTAGAACAACTTGTCTTCCAAAAGTAAGA8500.19905857005690733No Hit
CTTATATAAAATACCAGCCTTCCACAGAATATGCAAATAATGTCTAGTTT8200.19203297346666354No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT8010.18758342895950914No Hit
CTCTAGAACAACTTGTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA7670.17962108615723285No Hit
GAATATGCAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA7540.1765766609681272No Hit
CTGTAAGAGGGCCCACATATTGTTCACATCTGTTGACAATGTCTTCTTTC7230.16931687782487528No Hit
GTAGTAGGCTATGTCAATGTTAACATGGGCCTAAAATTCAGACAACTATT7140.16720919884780217No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6780.1587784829395096No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6730.15760755017446898No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA6720.15737336362146084No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6650.15573405775040397No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGAAGATTAAAATTGAT6480.15175288634926581No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAATATGCAAATAATGTCTAGT6330.14824008805414393No Hit
GCCTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC6180.14472728975902205No Hit
GGAACATATTGTGCAAAAATTGAAACAATGTTTTCGGAAACTTCCTGTAA6040.14144867801690827No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6000.14051193180487578No Hit
GTATACATGCATATAAAGGCATCAATGCTGGGTAACCACATTGTGTAAAA5980.1400435586988595No Hit
CTTTTACACAATGTGGTTACCCAGCATTGATGCCTTTATATGCATGTATA5960.13957518559284324No Hit
GTTGACAATGTCTTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5870.13746750661577012No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5790.1355940141917051No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGATTGCCGTG5790.1355940141917051No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCATTAAC5710.1337205217676401No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5670.13278377555560758No Hit
TCCTCAGGCCGTGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTGG5650.13231540244959136No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5610.13137865623755884No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCAGGATTCAGCGCCGACGGG5520.1292709772604857No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5470.12810004449544507No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5370.12575817896536381No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5350.12528980585934754No Hit
CCATACAGCAATAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5330.12482143275333131No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5110.11966932858715253No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5110.11966932858715253No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTACTGCCTCCACAAATCGGCAGT5110.11966932858715253No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGCACAATATGTT5000.11709327650406313No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCAATAAGGCA4940.11568815718601438No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT4940.11568815718601438No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4940.11568815718601438No Hit
GTTTGGGACTGTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG4910.11498559752699No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG4860.11381466476194936No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4830.11311210510292499No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4820.11287791854991687No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4780.11194117233788436No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAATATGCA4780.11194117233788436No Hit
TGTTAACATTGACATAGCCTACTACTAATTCCCTGGATGCTGGATCTTCC4770.11170698578487623No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCAAGC4730.11077023957284374No Hit
ATGTTAACATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCT4430.10374464298259994No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4430.10374464298259994No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4420.10351045642959182No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAAGCTTACAGACCACCAAATGCC4360.10210533711154306No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT4360.10210533711154306No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG4350.10187115055853492No Hit
CTGTAAGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC4300.10070021779349429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)