FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537890
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66551.2372418152410345No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA26400.4908066705088401No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13980.2599044414285449No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11980.2227221179051479No Hit
CTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11420.21231106731859675No Hit
CTACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT9570.17791741805945452No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT9450.1756864786480507No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9420.17512874379519977No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8800.1636022235029467No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8550.15895443306252208No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8400.1561657587982673No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC7900.14687017791741808No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7430.13813233188941978No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7070.1314395136552083No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6880.1279071929204856No Hit
CCTTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6730.12511851865623083No Hit
ACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATAT6650.12363122571529496No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6530.12140028630389112No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT5860.10894420792355315No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5820.10820056145308521No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5790.10764282660023425No Hit
CATTTAAACCCTAACAAAACCAAAAGATGGGGTTACTCTTTAAATTTCAT5750.1068991801297663No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5690.1057837104240644No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5650.10504006395359645No Hit
AGATAAAGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT5570.10355277101266058No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5560.10336685939504359No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5500.10225138968934168No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5440.10113591998363977No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTATACCTGTC5390.10020636189555486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)