FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537890
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66371.2338954061239287No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA24210.45009202625072037No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12850.23889642863782556No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11720.2178884158471063No Hit
CTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11340.21082377437766087No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9830.18275112011749614No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9760.18144973879417725No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT9540.17735968320660359No Hit
CTACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT8290.15412073100448045No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7930.147427912770269No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7700.14315194556507838No Hit
CCTTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7660.14240829909461042No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC7370.13701686218371786No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6600.12270166762721002No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6580.12232984439197606No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTTAC6560.12195802115674208No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT6400.11898343527487033No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6180.11489337968729667No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6140.11414973321682872No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5770.10727100336500028No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5580.10373868263027758No Hit
ACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATAT5460.10150774321887376No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5430.1009500083660228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)