FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382250
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21520.5629823413996076No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT11790.30843688685415305No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA10770.28175277959450623No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA10730.28070634401569655No Hit
CTCTTATACAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9530.24931327665140612No Hit
TTCAAGTACAGTGTTTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA9120.23858731196860694No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG9080.23754087638979726No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8860.23178548070634403No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8670.22681491170699805No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG7940.20771746239372138No Hit
CTTATACAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7480.19568345323741007No Hit
GTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT7350.19228253760627861No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7300.19097449313276652No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7100.18574231523871812No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTGTTTCTTCCAAAAGTAAGG7070.18495748855461086No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7070.18495748855461086No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT6930.18129496402877698No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC6790.1776324395029431No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6600.17266187050359713No Hit
GTTTTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAT6350.1661216481360366No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC6290.16455199476782212No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAGACGGGCAAC6270.16402877697841725No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6080.1590582079790713No Hit
ATTGAAAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG5980.15644211903204708No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC5960.15591890124264224No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5950.15565729234793982No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5950.15565729234793982No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT5850.15304120340091562No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5790.15147155003270113No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5560.14545454545454545No Hit
TAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTAC5370.14048397645519947No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5100.13342053629823414No Hit
GTCTAGGAGATCTCTGATGGAAGGAAAGAAGTCATGAGGCAAAAACGAGA5030.1315892740353172No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4970.13001962066710268No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4910.12844996729888816No Hit
ATACTAACATGGGTTTAAAGCTCAGGCAACTATTGTGGTTTCATATTTCT4870.12740353172007848No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGACAAGCCTTAGAGTCTCCTGA4870.12740353172007848No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4840.12661870503597122No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4830.1263570961412688No Hit
CTCTAAGGCTTGTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA4780.1250490516677567No Hit
CCTTTCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT4680.12243296272073251No Hit
GTTCAATGGTTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGCTATATG4590.12007848266841073No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCCTGTAT4570.11955526487900589No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC4530.11850882930019621No Hit
CCGATAAGCTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAG4510.11798561151079136No Hit
GTATACAGGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT4460.11667756703727927No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT4380.1145846958796599No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4340.11353826030085024No Hit
GGGATCTAGTAGTCAATTATGTCAATACTAACATGGGTTTAAAGCTCAGG4310.11275343361674298No Hit
CCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTC4290.11223021582733814No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG4140.10830608240680183No Hit
GTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4120.10778286461739699No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCAATCAGGCA4120.10778286461739699No Hit
GTATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4030.10542838456507522No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC3940.10307390451275344No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3940.10307390451275344No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC3880.10150425114453891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)