FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382250
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21500.5624591236102028No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT11150.2916939175931982No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA10780.2820143884892086No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10040.26265533028122956No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA9720.2542838456507521No Hit
CTCTTATACAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9660.2527141922825376No Hit
TTCAAGTACAGTGTTTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA9000.23544800523217788No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8920.23335513407455855No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8330.21792020928711578No Hit
GTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT8310.2173969914977109No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTGTTTCTTCCAAAAGTAAGG7840.2051013734466972No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7490.1959450621321125No Hit
GTTTTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAT7120.18626553302812296No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG7080.18521909744931328No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7040.1841726618705036No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT6970.18234139960758666No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6890.18024852844996728No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC6540.1710922171353826No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6410.16769130150425116No Hit
CTTATACAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6270.16402877697841725No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT6240.16324395029431No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6040.15801177240026162No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGACAAGCCTTAGAGTCTCCTGA6040.15801177240026162No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAGACGGGCAAC5810.15199476782210597No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5790.15147155003270113No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5660.14807063440156967No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC5550.14519293655984303No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC5470.14310006540222367No Hit
ATACTAACATGGGTTTAAAGCTCAGGCAACTATTGTGGTTTCATATTTCT5340.1396991497710922No Hit
GTCTAGGAGATCTCTGATGGAAGGAAAGAAGTCATGAGGCAAAAACGAGA5250.13734466971877043No Hit
ATTGAAAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG5210.13629823413996076No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5180.1355134074558535No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5080.1328973185088293No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4950.12949640287769784No Hit
CCTTTCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT4880.1276651406147809No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCAATCAGGCA4830.1263570961412688No Hit
CCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTC4730.1237410071942446No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4690.12269457161543493No Hit
GTTCAATGGTTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGCTATATG4670.12217135382603009No Hit
TAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTAC4600.12034009156311315No Hit
GGGATCTAGTAGTCAATTATGTCAATACTAACATGGGTTTAAAGCTCAGG4570.11955526487900589No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4570.11955526487900589No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4500.11772400261608894No Hit
GTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4450.11641595814257685No Hit
CTCTAAGGCTTGTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA4410.11536952256376717No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCCTGTAT4390.11484630477436232No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC4360.11406147809025506No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4300.11249182472204056No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC4290.11223021582733814No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4280.11196860693263572No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGACAACTTCCTGTTA4280.11196860693263572No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4160.10882930019620668No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT4110.10752125572269457No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4100.10725964682799215No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4040.10568999345977764No Hit
GTATACAGGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT3980.10412034009156311No Hit
GTATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT3980.10412034009156311No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC3850.10071942446043165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)