FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478044
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATACACCCGCCTTCCAGAGAGTGTGTAAATAATGTCTGGTTTGGA14810.30980411844934774No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13080.27361498104776966No Hit
CTCTAATACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA13020.2723598664558074No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGCCCACT12950.2708955660985181No Hit
CTCTTATATAATACACCCGCCTTCCAGAGAGTGTGTAAATAATGTCTGGT12890.26964045150655586No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT11260.2355431717582482No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT10820.22633899808385838No Hit
CCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG10750.2248746977265691No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAAACT10740.22466551196124204No Hit
AATCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10160.2125327375722737No Hit
TCCAGAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10110.21148680874563847No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC9740.20374693542853797No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAAC9470.19809891976470786No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC9340.19537950481545632No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCTTGTATT9280.19412439022349406No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9100.19035904644760734No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT8730.18261917313050682No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8720.18240998736517977No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8470.17718034323200377No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT8200.17153232756817366No Hit
GTATTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7850.16421082578172722No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7700.1610730393018216No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7570.15835362435257005No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT7550.15793525282191598No Hit
CTTATATAATACACCCGCCTTCCAGAGAGTGTGTAAATAATGTCTGGTTT7510.15709850976060782No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC7150.14956782220883433No Hit
ATATTAGTGTTAACATAACTGACTACTAGATCCCTGGATGTTTGATCTTC6810.14245550618771494No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6700.1401544627691175No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCACTGGAAAACACCCTCTTTTC6610.1382717908811741No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAGCATCTTGAGTCCCTTTTTACC6610.1382717908811741No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCGT6400.13387888980930626No Hit
GGGTAAAGGTGCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6160.12885843144145726No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6100.12760331684949502No Hit
GCTTTAGGGCATGGACATCGATCCTTATAAAGAATTTGGAGCTTCTGTGG6020.12592983072687872No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAATCTGTACAGCATCTTG5940.12425634460426238No Hit
TCCTCACGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5840.12216448695099195No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGCCTATGCCAAGTGTTTGCCG5760.12049100082837563No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG5670.11860832894043227No Hit
GGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTT5620.11756240011379705No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAATCTGTACAGCATCTTGAG5600.11714402858314296No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5580.1167256570524889No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5570.11651647128716185No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5560.11630728552183482No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5530.11567972822585368No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5460.11421542786856441No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5430.11358787057258327No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5400.11296031327660215No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5270.11024089832735062No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCACTGGAAAACACCCTCTTTTCCT5270.11024089832735062No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA5250.10982252679669655No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTTGTAACCTTTGGATAAAA5240.1096133410313695No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5180.10835822643940725No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA5130.10731229761277204No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5110.10689392608211797No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5080.10626636878613685No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5070.1060571830208098No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGAC5050.10563881149015572No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5010.10480206842884755No Hit
GTGCTGGTTCAGCCACAAGGGACACTATGGGAACAGGGACCTGGAGTTGC4980.10417451113286644No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC4980.10417451113286644No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4970.10396532536753939No Hit
GAATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4950.1035469538368853No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT4920.10291939654090418No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4890.10229183924492308No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA4850.10145509618361491No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC4830.10103672465296082No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4830.10103672465296082No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT4800.10040916735697969No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAGTGAGGGTTAAAGA4800.10040916735697969No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACC4790.10019998159165265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)