FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284289
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20880.7344638730306132No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6350.22336425257396525No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6240.21949495056087293No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5450.19170632701230086No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA5260.18502298717150506No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT4990.17552560950300575No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4750.16708349601989525No Hit
ATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC4530.15934489199371063No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4370.1537168163383036No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC4350.15301330688137776No Hit
TCCCTAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCG4280.1505510237821372No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG4210.14808874068289662No Hit
CTCTATGACCGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4070.1431641744844155No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC3910.13753609882900852No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3890.13683258937208265No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3670.12909398534589803No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT3620.12733521170358333No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3620.12733521170358333No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT3490.12276240023356515No Hit
ATATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTTGATCTTC3430.12065187186278753No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT3400.1195966076773987No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCAACCAAACTCTGCAAG3320.1167825698496952No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC3290.11572730566430639No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGCA3290.11572730566430639No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA3280.11537555093584345No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3230.11361677729352876No Hit
TACATGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATAC3200.11256151310813996No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT3160.11115449419428822No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT3150.11080273946582526No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3060.10763694690965883No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT3060.10763694690965883No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGG3050.1072851921811959No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG3020.10622992799580709No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT3000.10552641853888121No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2960.10411939962502946No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA2940.1034158901681036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)