FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573569
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69251.2073525591515581No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA38300.6677487800072877No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT22750.39663928838552986No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT22090.3851323903488508No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT16550.2885441856167262No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT15860.27651424676019803No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTT14980.2611717160446258No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC14740.25698738948583344No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG14510.2529774098669907No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT12900.2249075525350917No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT12700.221420613736098No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT12340.21514412389790938No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12030.2097393687594692No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT11860.2067754707803246No Hit
CACTTACAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGG11460.19980159318233726No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT11270.1964890013232933No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT10800.18829469514565814No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG10550.18393602164691608No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTAGTCTGATGTGTT10200.17783387874867715No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTAGT10110.17626475628913No Hit
ATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT9930.17312651137003568No Hit
CTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC9760.1701626133908911No Hit
CTTACAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTT9750.1699882664509414No Hit
GTTTGTAGGCCCACTTACAGTTAATGAGAAAAGAAGATTGAAATTGATTA9650.16824479705144457No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTTACAGT9530.16615263377204836No Hit
CTCATAAGGTGGGGAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC9420.16423481743260182No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA9400.16388612355270246No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC9320.162491348033105No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA9180.16005049087380943No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG9010.1570865928946648No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8990.15673789901476542No Hit
GGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCATTAAC8840.1541226949155202No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8730.15220487857607368No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8480.1478462050773316No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT8350.1455796948579857No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT8300.14470796015823728No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT8000.13947755195974676No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACACTTCTT7910.13790842950019963No Hit
GGCATAATCAATTTCAATCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC7890.13755973562030024No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7460.13006281720246388No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCATGTATT7260.1265758784034702No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7030.12256589878462748No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA7000.12204285796477844No Hit
GTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTATCC6980.12169416408487907No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCA6860.11960200080548286No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAA6800.11855591916578477No Hit
GGGTTACTCTCTGAATTTTATGGGTTATGTCATTGGAAGTTATGGGTCCT6780.1182072252858854No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6770.1180328783459357No Hit
GTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA6660.11611506200648919No Hit
ATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAA6650.11594071506653952No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC6500.11332551096729425No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGTCTTTATTC6500.11332551096729425No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6500.11332551096729425No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTTCACCAAACTCTGCAAG6460.11262812320749552No Hit
TCCTTGGACTCATAAGGTGGGGAACTTTACTGGTCTTTATTCTTCTACTG6200.10809510276880376No Hit
TTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCT6190.10792075582885406No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGAT6160.10739771500900502No Hit
CAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTAT6140.10704902112910566No Hit
GTTTTATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAAC6040.10530555172960882No Hit
GAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATT6040.10530555172960882No Hit
ATGTAAGACCTTGGGCAACATTCGGTGGGCGTTCACGGTGGTTTCCATGC5880.10251600069041387No Hit
CAATGTTAGTATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGTCTTT5830.10164426599066548No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCCTATCAACACTTCCGGA5820.10146991905071578No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACAC5790.10094687823086675No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG5740.10007514353111832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)