FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573569
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131102.2856883827403505No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA34050.5936513305286722No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT22710.3959419006257312No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT21030.36665161471418434No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC16410.2861033284574306No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT15580.27163253244160684No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG14810.2582078180654812No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14570.25402349150668885No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAATCAATTT14540.25350045068683974No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT12910.22508189947504134No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT12260.21374934837831194No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT12120.21130849121901635No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT11990.20904198099967047No Hit
CACTTACAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGG10160.1771364909888784No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT10080.17574171546928094No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG10020.17469563382958284No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTAGTCTGATGTGTT9940.17330085830998537No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9920.172952164430086No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT9770.17033696033084075No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA9580.16702436847179677No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTAGT9290.16196830721325595No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9160.15970179699391007No Hit
GTTTGTAGGCCCACTTACAGTTAATGAGAAAAGAAGATTGAAATTGATTA9120.15900440923411133No Hit
CTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC9030.15743528677456417No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA8950.1560405112549667No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC8640.15063575611652652No Hit
ATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT8630.15046140917657685No Hit
CTCATAAGGTGGGGAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC8620.15028706223662716No Hit
CTTACAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTT8360.14575404179793539No Hit
GGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCATTAAC8320.14505665403813664No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTTACAGT7860.13703669480045122No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT7700.13424714376125627No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG7550.13163193966201103No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT7510.1309345519022123No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCATGTATT7440.12971412332256452No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7420.12936542944266516No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA7350.12814500086301736No Hit
ATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATAA7280.12692457228336956No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG7130.12430936818412432No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA7080.12343763348437589No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACACTTCTT7010.12221720490472811No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6910.12047373550523129No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCA6830.11907895998563381No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAA6800.11855591916578477No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6720.11716114364618728No Hit
GGCATAATCAATTTCAATCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC6670.11628940894643888No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTTCACCAAACTCTGCAAG6570.11454593954694203No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6560.11437159260699237No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6490.11315116402734458No Hit
GGGTTACTCTCTGAATTTTATGGGTTATGTCATTGGAAGTTATGGGTCCT6480.11297681708739489No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCCTATCAACACTTCCGGA6480.11297681708739489No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGAT6300.10983857216830058No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6250.10896683746855218No Hit
GTTTTATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAAC6220.10844379664870311No Hit
GTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA6160.10739771500900502No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATTTACACACTCTATGGA6160.10739771500900502No Hit
GTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTATCC6150.10722336806905533No Hit
TCCTTGGACTCATAAGGTGGGGAACTTTACTGGTCTTTATTCTTCTACTG6100.1063516333693069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)