FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270571
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19080.7051753513865122No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA6920.2557554209431166No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACT5160.19070779943157248No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5080.18775108936286594No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4570.16890206267486169No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3720.1374870181948546No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG3680.1360086631605013No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT3660.13526948564332467No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC3660.13526948564332467No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT3650.13489989688473636No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC3250.12011634654120361No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT3230.11937716902402697No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC3130.11568128143814378No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3000.11087662757649563No Hit
CACTAATATGGGCTTAAAATTCAGGCAACTTTTGTGGTTTCACATTTCTT2940.10865909502496574No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT2930.10828950626637741No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC2880.1064415624734358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)