FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383683
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT22950.5981500353156122No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA17640.45975453694847046No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC14830.3865169944980622No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGCCATGT14450.3766129851987187No Hit
ATTCTTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT13620.3549805438343633No Hit
GTACGGGACCGTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10930.2848705832679582No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA10230.26662635561127285No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT10110.26349877372726965No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC10050.26193498278526806No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9970.2598499281959326No Hit
CCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA9810.2556798190172616No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT9780.25489792354626084No Hit
GGTTTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG9730.25359476442792617No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9690.2525522371332585No Hit
ATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGAGA9530.2483821279545875No Hit
AAGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC9510.24786086430725363No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCTGA9020.2350899049475739No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8820.22987726847423523No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA8580.22362210470622887No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT8320.21684567729088858No Hit
CTACTTGTGGTTTCCCTCTTATATAGAATACCAGCCTTCCAAAGAGTATG8080.21059051352288216No Hit
CCATGTAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAG8000.2085054589335467No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7840.20433534975487577No Hit
GTATACAGGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAAT7180.18713364939285818No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7130.1858304902745235No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7070.1842666993325219No Hit
GGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAATGGAGCAG7020.18296354021418723No Hit
CTCTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGC7000.18244227656685336No Hit
TTACTGGGCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAA6900.179835958330184No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6870.17905406285918324No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6640.17305953091484377No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6580.17149573997284215No Hit
AATACACATCGTTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTT6540.17045321267817443No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA6360.16576183985216963No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6110.1592460442604963No Hit
ATGCAGGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCC6060.15794288514216162No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5720.14908140313748589No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5500.14334750301681337No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT5500.14334750301681337No Hit
ATGGCGACCACCGTGAACGCCCATCAGATCCTGCCCAAGGTCTTATATAA5420.14126244842747787No Hit
GTTTGGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG5400.14074118478014402No Hit
GAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA5240.13657107560147308No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTC5210.13578918013047228No Hit
AAGTAAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5170.13474665283580456No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5120.13344349371746989No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5110.13318286189380296No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5060.1318797027754683No Hit
AATCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT5050.13161907095180136No Hit
GACAACTCTGTCGTCCTCTCGCGGAAATATACATCGTTTCCATGGCTGCT4940.12875212089146507No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAATCTCCTCC4940.12875212089146507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4860.12666706630212962No Hit
CCATTTACACAATGTGGATATCCTGCATTAATGCCTTTGTATGCCTGTAT4720.12301822077079255No Hit
GGTCGCCATGTAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG4690.12223632529979175No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA4670.12171506165245789No Hit
CTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTG4650.12119379800512402No Hit
CTACTTGTGGTTTCCCTCTTATATAGAACACCAGCCTTCCAAAGAGTATG4600.11989063888678936No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4560.11884811159212161No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4520.11780558429745389No Hit
CCTGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4510.11754495247378695No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4430.11545989788445149No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4400.11467800241345069No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT4400.11467800241345069No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT4370.11389610694244989No Hit
GACCTAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCGAGGCGA4370.11389610694244989No Hit
GTCCTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA4310.11233231600044828No Hit
ACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4240.11050789323477975No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAATCT4220.1099866295874459No Hit
TCCTAATACAAAGATCATTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT4150.10816220682177737No Hit
CATCCAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCG4130.10764094317444349No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4060.10581652040877496No Hit
ATGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAAT4050.10555588858510803No Hit
ATTACAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAATAA4040.10529525676144108No Hit
ATCTCAATCTCGGGAACCTCAATGTTAGTATTCCTTGGACTCATAAGGTG3970.10347083399577255No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC3970.10347083399577255No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT3960.10321020217210562No Hit
TGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGATA3960.10321020217210562No Hit
CTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT3930.10242830670110481No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3900.10164641123010401No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA3880.10112514758277016No Hit
TCATTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAG3870.10086451575910321No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGACGATGGGATGGGAATACAGGTG3840.10008262028810243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)