FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383683
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT22400.5838152850139308No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA17250.44958989582546005No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC13450.35054980283202536No Hit
ATTCTTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT12870.3354331570593433No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGCCATGT11460.2986840699223056No Hit
GTACGGGACCGTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11160.29086511521229763No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG10150.26454130102193735No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA9840.25646171448826244No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT9770.2546372917225939No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9430.24577580971791818No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT9230.24056317324457951No Hit
AAGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC9180.23926001412624484No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC9020.2350899049475739No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA8900.2319623230635707No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT8840.23039853212156908No Hit
ATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGAGA8480.2210157864695595No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT8440.2199732591748918No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCTGA8300.21632441364355473No Hit
CTACTTGTGGTTTCCCTCTTATATAGAATACCAGCCTTCCAAAGAGTATG8020.20902672258088056No Hit
GGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAATGGAGCAG8020.20902672258088056No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT7640.1991227132815371No Hit
CCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA7550.1967770268685347No Hit
GGTTTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG7510.19573449957386696No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7330.19104312674786217No Hit
GTATACAGGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAAT7160.1866123857455243No Hit
TTACTGGGCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAA7070.1842666993325219No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6920.1803572219775179No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6640.17305953091484377No Hit
CCATGTAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAG6580.17149573997284215No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC6480.16888942173617283No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6150.16028857155516404No Hit
AATACACATCGTTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTT6070.15820351696582854No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6060.15794288514216162No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5970.15559719872915923No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA5960.1553365669054923No Hit
ATGCAGGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCC5860.15273024866882295No Hit
GTTTGGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG5780.1506451940794875No Hit
ATGGCGACCACCGTGAACGCCCATCAGATCCTGCCCAAGGTCTTATATAA5740.14960266678481976No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5700.14856013949015204No Hit
CTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTG5540.1443900303114811No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5470.14256560754581257No Hit
CTCTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGC5450.1420443438984787No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5390.1404805529564771No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5090.1326615982464691No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT5030.1310978073044675No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5010.13057654365713364No Hit
GACAACTCTGTCGTCCTCTCGCGGAAATATACATCGTTTCCATGGCTGCT4960.12927338453879894No Hit
CCATTTACACAATGTGGATATCCTGCATTAATGCCTTTGTATGCCTGTAT4940.12875212089146507No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTC4900.12770959359679734No Hit
AATCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT4890.12744896177313042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4850.1264064344784627No Hit
GAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA4790.12484264353646109No Hit
AAGTAAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4780.12458201171279416No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4730.12327885259445948No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA4620.12041190253412322No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4580.11936937523945548No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAATCTCCTCC4530.11806621612112084No Hit
ATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCTTGAGG4340.11311421147144908No Hit
CCTGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4340.11311421147144908No Hit
GACCTAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCGAGGCGA4250.11076852505844667No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4200.10946536594011202No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG4180.10894410229277815No Hit
CATCCAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCG4180.10894410229277815No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA4120.10738031135077655No Hit
CTACTTGTGGTTTCCCTCTTATATAGAACACCAGCCTTCCAAAGAGTATG4040.10529525676144108No Hit
GTCCTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA4040.10529525676144108No Hit
ATCCCGCGGACGACCCCTCTCGGGGCCGCTTGGGAATCTATCGTCCCCTT4010.10451336129044028No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGCGCTACTGTCGA4010.10451336129044028No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT3930.10242830670110481No Hit
GTCCTCAAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACC3920.10216767487743789No Hit
GGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3900.10164641123010401No Hit
ACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG3870.10086451575910321No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT3840.10008262028810243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)