FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476668
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA34170.7168511416751282No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24760.5194391064640379No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14910.3127963278424396No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14150.2968523164970168No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13090.27461461646261126No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT12670.26580345229803554No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAAT12080.25342586454303623No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10980.2303490060167664No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10650.22342594845888544No Hit
CTCTAAGACTACGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10430.21881057675363144No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10400.2181812078847332No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10320.21650289090100447No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT10210.2141952050483775No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATT9770.20496446163786952No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9510.19950993144075124No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAATCTAGCAGG9310.19531413898142944No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9190.19279666350583635No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9030.1894400295383789No Hit
GGTAAATATTTGGTAACATTTGGATAAAATCTAGCAGGCATAATCAATTG8480.177901600275244No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG8400.17622328329151526No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8270.1734960181929561No Hit
CAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTAT8230.17265685970109174No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC7740.16237716817575334No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA7480.15692263797863504No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7290.15293663514227934No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7110.1491604219288897No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7050.14790168419109317No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6850.14370589173177137No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6660.13971988889541567No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6650.1395100992724496No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6580.13804157191168698No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA6500.13636325492795823No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTATCCAAATGTTA6450.1353143068131278No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6410.13447514832126342No Hit
ATCCAAATGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT6400.13426535869829734No Hit
CCTCTATGTATCCCTCATGTTGCTGTACCAAACCTTCGGACGGAAATTGC6170.12944019737007728No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6150.12902061812414511No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAA6040.12671293227151811No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATT5970.1252444049107555No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA5900.12377587754999286No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5900.12377587754999286No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5790.12146819169736589No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA5730.12020945395956933No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5670.11895071622177279No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5650.11853113697584063No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5510.11559408225431536No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5450.11433534451651883No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5370.11265702753279012No Hit
CTACAAACATTGCCAGAAAATCCGCCTCCGGCCTCTACCAATCGCCAGTC5360.11244723790982403No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTATCC5340.11202765866389186No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5220.10951018318829876No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5180.10867102469643442No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5050.10594375959787526No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATCAGAGGACAAACGGGCAAC4990.10468502186007872No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4930.10342628412228219No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4820.10111859826965519No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4780.10027943977779083No Hit
ATCCTATGCTGGGGGGAACTAATGACTCTAGCTACCTGGGTGGGTGGTAA4770.10006965015482475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)