FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476668
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32610.6841239604924182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24670.5175509998573431No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14880.31216695897354135No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14220.2983208438577794No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAAT12180.2555237607726971No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12030.2523769164282058No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11550.2423070145258335No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT11350.23811122206651172No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11050.231817533377529No Hit
CTCTAAGACTACGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11020.23118816450863072No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10440.21902036637659753No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10350.21713225976990272No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAATCTAGCAGG9690.20328614465414085No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATT9580.20097845880151385No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9310.19531413898142944No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9220.1934260323747346No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9180.19258687388287024No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9060.19006939840727718No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8780.18419528896422668No Hit
CAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTAT8290.17391559743888826No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8050.16888064648770212No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG7990.1676219087499056No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7850.16468485402838035No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA7730.16216737855278726No Hit
GGTAAATATTTGGTAACATTTGGATAAAATCTAGCAGGCATAATCAATTG7360.15440516250304195No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7140.14978979079778798No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6770.14202757474804267No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC6720.1409786266332122No Hit
CCTCTATGTATCCCTCATGTTGCTGTACCAAACCTTCGGACGGAAATTGC6550.1374122030427887No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6270.1315380935997382No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6260.1313283039767721No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6250.131118514353806No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA6140.12881082850117903No Hit
ATCCAAATGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT6140.12881082850117903No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT6050.1269227218944842No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA6040.12671293227151811No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6010.12608356340261986No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6010.12608356340261986No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAA5970.1252444049107555No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTATCCAAATGTTA5930.12440524641889113No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5920.12419545679592506No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5870.1231465086810946No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA5830.12230735018923024No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5760.12083882282846763No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5550.11643324074617972No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATT5360.11244723790982403No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5350.11223744828685793No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5340.11202765866389186No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5170.10846123507346833No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTATCC5040.10573396997490916No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT5000.10489481148304479No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4930.10342628412228219No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4790.10048922940075693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)