FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339178
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTAGA20560.6061713908331319No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11440.33728602680598385No Hit
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACGTAGCGCC9910.29217696902511364No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9090.2680008726981113No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7900.23291604997965668No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7250.21375207118386214No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6960.20520198833650768No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTCACTCTTTACATTTTAT6500.19163978795794537No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6160.18161555289552977No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6050.1783724180223953No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5870.1730654700481753No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5590.16481021764383302No Hit
GTGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTAGATGTTCTGGAT5580.16451538720082082No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT5300.15626013479647854No Hit
GTGTAAAAGGGGCAGCAAACCCCAATAGACCCACAATACGTTGACAGACT5270.15537564346744187No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCAAAACC5240.1544911521384052No Hit
CCTCCAGCTTATAGACCAGTAAATGCCCCTATCTTATCAACACTTCCGGA5120.1509531868222585No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4960.14623589973406292No Hit
ATCCCAGAGTGAGCGGCCTGATTGCTGGTGGCTCCAGTTCAGGAACAGTA4670.13768581688670845No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTC4350.1282512427103173No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCTCTGGATGTTGGATCTTC4350.1282512427103173No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4290.12648226005224394No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATA4270.1258925991662195No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCCGTG4240.12500810783718286No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT4210.12412361650814617No Hit
ATACCACATCGTCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4170.12294429473609728No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCGCCATGT4150.12235463385007281No Hit
GATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTTG4140.1220598034070606No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA4140.1220598034070606No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCATTGTATGCATGTATT4050.1194063294199506No Hit
AGTCTGTACACCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3980.11734251631886501No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT3980.11734251631886501No Hit
GTCATAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3920.11557353366079169No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3870.11409938144573055No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3870.11409938144573055No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTGTTGTGGTTTCACATTTCTT3840.11321489011669389No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAGT3760.1108562465725961No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC3750.11056141612958388No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAG3740.11026658568657165No Hit
CCTCTATGTATCCCTCATGTTGTTGTACCAAACCTTCGGACGGAAATTGC3740.11026658568657165No Hit
GTGTAAATGTATGTCAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA3710.10938209435753499No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA3660.10790794214247386No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3620.10672862037042496No Hit
ATGTAAAGAGTGACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC3590.1058441290413883No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3530.10407514638331496No Hit
CTTTAACCCTCATTGGAAAACACCATCTTTTCCTGACATACATTTACACC3460.10201133328222939No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCT3460.10201133328222939No Hit
ATCAAGGTATGTTGCCCGTTTGTCCCATGATTCCAGGATCTTCAACCACA3420.10083201151018048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)