FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339178
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTAGA19360.570791737671665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11340.3343377223758616No Hit
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACGTAGCGCC9870.29099764725306476No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9320.2747819728873925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8800.25945078985075687No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7100.20932961453867882No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6750.19901054903325097No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6500.19163978795794537No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6070.17896207890841978No Hit
CCTCCAGCTTATAGACCAGTAAATGCCCCTATCTTATCAACACTTCCGGA5940.17512928314926088No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5900.17394996137721197No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTCACTCTTTACATTTTAT5830.17188614827612642No Hit
GTGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTAGATGTTCTGGAT5610.16539987852985746No Hit
GTGTAAAAGGGGCAGCAAACCCCAATAGACCCACAATACGTTGACAGACT5600.16510504808684526No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5150.1518376781512952No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4970.14653073017707519No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCAAAACC4910.1447617475190018No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT4830.14240310397490402No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4790.14122378220285514No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTC4770.1406341213168307No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTGTTGTGGTTTCACATTTCTT4560.13444268201357398No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATA4470.131789208026464No Hit
ATCCCAGAGTGAGCGGCCTGATTGCTGGTGGCTCCAGTTCAGGAACAGTA4390.12943056448236617No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4270.1258925991662195No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4230.12471327739417061No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCATTGTATGCATGTATT4220.12441844695115839No Hit
AGTCTGTACACCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4160.12264946429308506No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA4070.11999599030597502No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT4060.11970115986296281No Hit
GTCATAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3980.11734251631886501No Hit
CCTCTATGTATCCCTCATGTTGTTGTACCAAACCTTCGGACGGAAATTGC3960.11675285543284059No Hit
ATACCACATCGTCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3950.11645802498982834No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCCGTG3910.11527870321777944No Hit
GATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTTG3840.11321489011669389No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCTCTGGATGTTGGATCTTC3820.11262522923066944No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3800.112035568344645No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAGT3790.11174073790163276No Hit
GTGTAAATGTATGTCAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA3770.11115107701560832No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC3770.11115107701560832No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3750.11056141612958388No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACACCACCTTGAGTCCCTTTTTACC3720.10967692480054721No Hit
CTTTAGGACATGGACATTGATCCGTATAAAGAATTTGGAGCTTCTGTCGA3700.10908726391452275No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCT3670.10820277258548609No Hit
CCCCAATAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT3520.10378031594030274No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCGCCATGT3500.10319065505427828No Hit
GTATAAAGAATTTGGAGCTTCTGTCGAGTTACTCTCTTTTTTGCCTTCTG3480.10260099416825384No Hit
ATCAAGGTATGTTGCCCGTTTGTCCCATGATTCCAGGATCTTCAACCACA3460.10201133328222939No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAG3440.10142167239620495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)